The CD3/CD28 Streptamer® approach is based on our Strep-tag® technology and facilitates T cell activation and expansion. Low affinity, Fab fragments fused to a Twin-Strep-tag® (Fab-Streps) against CD3 and CD28 are multimerized on a Strep-Tactin® backbone. The resulting CD3/CD28 Streptamer® complex stimulates the T cell receptor/CD3 complex (TCR/CD3; signal 1) and the co-stimulatory molecule CD28 (signal 2). Providing these two signals leads to efficient T cell activation and proliferation.
For in vitro stimulation and activation, the soluble CD3/CD28 Streptamers are added to T cells. The addition of biotin reverses the multimerization and causes the dissociation of low affinity Fab fragments from the cells. Consequently, the termination of stimulation is possible at any point in time.
- Product Reviews
- Product Publications
- Liying An, et al. (2022). Concurrent Stimulation of Monocytes with CSF1 and Polarizing Cytokines Reveals Phenotypic and Functional Differences with Classical Polarized Macrophages. Journal of Leukocyte Biology, 112(3), 437-447. doi: 10.1002/JLB.3A0721-383R
- Fabian Mohr, et al. (2018). Efficient Immunoaffinity Chromatography of Lymphocytes Directly from Whole Blood. Scientific Reports, 8: 16731. doi: 10.1038/s41598-018-34589-z
- Sarah Matko, et al. (2017). Enumeration of WT1-Specific CD8+ T Cells for Clinical Application Using an MHC Streptamer Based No-Wash Single-Platform Flow-Cytometric Assay. Cytometry Part A, 91(10), 1001-1008. doi: 10.1002/cyto.a.23146
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