Vimentin

WB of crude extract of human embryonic kidney stained with Vimentin, showing a single strong clean band at ~50kDa and view of mixed neuron/glial cultures stained with Vimentin (green) and GFAP antibody (RA22101, red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the imate which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin).

hN2 Primary Neurons stained with our chicken polyclonal antibody to Vimentin, in red. Islands of Hn2 cells form after 4 days in culture forming beautiful flower like structures. Vimentin is a well established marker of early differentiating neuronal lineage cells. Taken with a 10X objective lens. Blue staining is the nuclear DNA

Human HeLa cells stained with Vimentin and our 39C7 nuclear pore complex marker.