Product Details
Catalog Number: CH22112
Applications: WB, IF
Type: Chicken IgY
Dilutions: ICC 1:2000; IHC 1:2000; WB 1:10,000
Immunogen: Mixture of 3 MBP peptides which are the different forms of the protein which are alternate transcription from a single gene.
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (polar packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Mouse, Rat, Bovine
Entrez: 4155
UniProt: P02686
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$295.00Add to Cart

Myelin basic protein is reported to account for about 30 of the proteins in myelin found in the central nervous system. It can induce experimental allergic encephalomyelitis (EAE), a T-lymphocyte mediated disease due to delayed-type hypersensitivity; though each animal species appears to respond to a different fragment of the 170 amino acid polypeptide. Four different isoforms have been identified through cDNA cloning. All four of these variants are identical except for the insertion or deletion of two peptide fragments encoded by exons 2 and 5. Myelin basic protein is reported to be expressed in oligodendrocytes, myelin of white matter in the brain and spinal cord and in peripheral nerves, though it is expressed less abundantly in gray matter.

MBP has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. In vitro, MBP is suitable as a substrate for numerous protein kinases, including the ERK and p38 MAP kinases that phosphorylate MBP at T98. 

Images

Rat Oligodendrocytes were cultured on Lamnin+Fibronectin for 2 days and then differentiated with T3 for 2 days. Cells were rinsed with PBS, fixed with 4% PFA for 10 min, and then rinsed in PBS. For IHC, cells were blocked in 5% NDS in PBSTx 0.1%, incubated in MBP at 1:500 (CH22112) 4C O/N, washed 3x in PBS, incubated in secondary 1:1000 2h RT, washed 3x and then mounted. Co-stained with Olig2. Courtesy of Katherine Given, UC Dever

Rat Oligodendrocytes were cultured on Lamnin+Fibronectin for 2 days and then differentiated with T3 for 2 days. Cells were rinsed with PBS, fixed with 4% PFA for 10 min, and then rinsed in PBS. For IHC, cells were blocked in 5% NDS in PBSTx 0.1%, incubated in MBP at 1:500 (CH22112) 4C O/N, washed 3x in PBS, incubated in secondary 1:1000 2h RT, washed 3x and then mounted. Co-stained with Olig2. Courtesy of Katherine Given, UC Dever

Shows rat mixed neuron/glial cultures stained with chicken Myelin Basic Protein (green). Blue is a DNA stain. Note that the Myelin Basic Protein antibody stains an oligodendrocyte and some membrane shed from this cell. Other cells in the field include neurons, astrocytes, microglia and fibroblasts, all of which are completely negative. specifically, and that the staining is concentrated in the cell bodies. Protocol on data-sheet.

Shows rat mixed neuron/glial cultures stained with chicken Myelin Basic Protein (green). Blue is a DNA stain. Note that the Myelin Basic Protein antibody stains an oligodendrocyte and some membrane shed from this cell. Other cells in the field include neurons, astrocytes, microglia and fibroblasts, all of which are completely negative. specifically, and that the staining is concentrated in the cell bodies. Protocol on data-sheet.

Blots of whole tissue homogenate of rat spinal cord with chicken antibody to Myelin Basic Protein. The antibody stains a prominent ~20kDa band at the dye front on this 12% acrylamide gel. Protocol on data-sheet.

Blots of whole tissue homogenate of rat spinal cord with chicken antibody to Myelin Basic Protein. The antibody stains a prominent ~20kDa band at the dye front on this 12% acrylamide gel. Protocol on data-sheet.