Images
Hippocampal Neurons stained with Tau and UCHL

E18 Primary Rat Hippocampal Neurons (PC35101) fixed and immunostained with Tua/MAPT+(CH22113). The cells were also stained in green with our monoclonal antibody to Ubiquitin C-terminal Hydrolase 1 (UCHL1,MO22109). Since the perikarya contain both UCHL1 and Tau, the red and green signals superimpose, giving a yellow color, while the processes, which contain relatively much more tau, appear red. Blue stain is DAPI and reveals cell nuclei of some non neuronal cells in these cultures.

Hippocampal Neurons stained with Tau and UCHL

E18 Primary Rat Hippocampal Neurons (PC35101) fixed and immunostained with Tua/MAPT+(CH22113). The cells were also stained in green with our monoclonal antibody to Ubiquitin C-terminal Hydrolase 1 (UCHL1,MO22109). Since the perikarya contain both UCHL1 and Tau, the red and green signals superimpose, giving a yellow color, while the processes, which contain relatively much more tau, appear red. Blue stain is DAPI and reveals cell nuclei of some non neuronal cells in these cultures.

Embryonic day-18 primary rat hippocampal cells (PC 35101) grown over pLL-coated 6-well plate for 48 hours. Green: anti-rat TAU/MAPT (CH22113), blue: DAPI. Scale bar = 25 um.

Embryonic day-18 primary rat hippocampal cells (PC 35101) grown over pLL-coated 6-well plate for 48 hours. Green: anti-rat TAU/MAPT (CH22113), blue: DAPI. Scale bar = 25 um.

Embryonic day-18 primary rat hippocampal cells (PC 35101) grown over pLL-coated 6-well plate for 48 hours. Green: anti-rat TAU/MAPT (CH22113), blue: DAPI. Scale bar = 25 um.

Embryonic day-18 primary rat hippocampal cells (PC 35101) grown over pLL-coated 6-well plate for 48 hours. Green: anti-rat TAU/MAPT (CH22113), blue: DAPI. Scale bar = 25 um.

Blots of crude rat brain extract. Tau protein is expressed as up to 8 different isoforms of different molecular weight and so appears as multiple closely spaced bands covering the region of the blot from 50kDa to 70kDa. Lane 11 was probed with CPCA-Tau while lane 12 was probed with tau monoclonal antibody. Protocol on Data-sheet.

Blots of crude rat brain extract. Tau protein is expressed as up to 8 different isoforms of different molecular weight and so appears as multiple closely spaced bands covering the region of the blot from 50kDa to 70kDa. Lane 11 was probed with CPCA-Tau while lane 12 was probed with tau monoclonal antibody. Protocol on Data-sheet.

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