Product Details
Catalog Number: CH22123
Applications: WB, IHC, IF
Type: Chicken IgY
Dilutions: IF/IHC: 1:500-1:1.000 Western Blot: 1:5,000
Immunogen: Recombinant construct, amino acids 317-630 of the human protein expressed in E coli.
Storage: Store at 4°C short term. Store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Species Reactivity: Human, Mouse, Rat
Entrez: 10763
UniProt: P48681
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
100 ul of 1 mg/ml$275.00Add to Cart

Nestin belongs to the class IV intermediate filament protein family. Nestin is expressed in radial glia and other dividing cells in the developing CNS, PNS and muscles. Nestin is also found to be expressed in brain tumors, particularly gliomas. Nestin is also a marker of stem cells in the pancreas and heart. The nestin amino acid sequence is relatively poorly conserved in protein sequence across species boundaries, so that the mouse and human proteins have an overall identity of only 62%. As a result, antibodies to the human protein often fail to recognize the rodent homologue and vice versa. However this antibody works well on both human and rodent cells and tissues.

Images
Immunoflueorescent

Immunofluorescent analysis of rat embryonic (E18) brain stained with chicken Nesting (CH22123), dilution 1:2,000 in red. Nuclear DNA is stained blue with Hoechst.

Immunoflueorescent

Immunofluorescent analysis of rat embryonic (E18) brain stained with chicken Nesting (CH22123), dilution 1:2,000 in red. Nuclear DNA is stained blue with Hoechst.

Western Blot

Western blot analysis of different cell lysates using rabbit pAB to nestin, CH22123, dilution 1:3,000 in green: [1] protein standard, [2] rat cortical neuron-glial cell culture , and [3] SH-SY5Y cells. High molecular weight band corresponds to nestin protein detected only in human cells.

Western Blot

Western blot analysis of different cell lysates using rabbit pAB to nestin, CH22123, dilution 1:3,000 in green: [1] protein standard, [2] rat cortical neuron-glial cell culture , and [3] SH-SY5Y cells. High molecular weight band corresponds to nestin protein detected only in human cells.