Product Details
Catalog Number: CH22124
Applications: WB, IHC, IF
Type: Chicken IgY
Dilutions: Immunofluorescence: 1:1,000-5,000 Immunohistochemistry: 1:1,000-5,000 Western Blot: 1:1,000-5,000
Immunogen: Recombinant Aequoria coerulescens GFP purified from E. coli
Storage: Store at 4°C short term. Store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: liquid
Species Reactivity: Not Applicable
Entrez: 7011691
UniProt: P42212
Downloads: Datasheet (pdf)
Product Sizes
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100 ul of 1 mg/ml$325.00Add to Cart

GFP (green fluorescent protein) is 27kDa protein; which was original isolated from the jellyfish (Aequoria victoria). This antibody was made against a recombinant GFP, from the Aequoria species, referred to as AcGFP. This antibody can be used to verify the expression, size and stability of both AcGFP and eGFP fusion proteins in western blotting experiments and to amplify GFP signals in tissues of transgenic animals.

Images
Immunofluorescent

Immunofluorescent analysis of transfected HEK293 cells with a GFP-construct in green stained with chicken antibody to GFP, CH22124, dilution 1:1,000 in red. The blue is Hoechst staining of nuclear DNA. The CH22124 antibody reveals GFP protein expressed only in transfected cells, as a result transfected cells are appeared express both red and green signals and so appear an orange-golden color. Untransfected cells show neither signal.

Immunofluorescent

Immunofluorescent analysis of transfected HEK293 cells with a GFP-construct in green stained with chicken antibody to GFP, CH22124, dilution 1:1,000 in red. The blue is Hoechst staining of nuclear DNA. The CH22124 antibody reveals GFP protein expressed only in transfected cells, as a result transfected cells are appeared express both red and green signals and so appear an orange-golden color. Untransfected cells show neither signal.

Western Blot

Western blot analysis of HEK293 cell lysates using goat pAb to GFP, GPCA-GFP, dilution 1:1,000, in green: [1] protein standard, [2] non-transfected control cells, [3] cells transfected with a GFP construct and [4] cells transfected with an mCherry construct.

Western Blot

Western blot analysis of HEK293 cell lysates using goat pAb to GFP, GPCA-GFP, dilution 1:1,000, in green: [1] protein standard, [2] non-transfected control cells, [3] cells transfected with a GFP construct and [4] cells transfected with an mCherry construct.