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Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with chicken pAb to ankyrin 3, CH22125, dilution 1:2,000 in green, and costained with mouse mAb to FOX3/NeuN dilution 1:2,000 in red. The blue is Hoechst staining of nuclear DNA. The CH22125 antibody stains the axonal initial segments, while the FOX3/NeuN antibody reveals perikarya and nuclei of neurons.
Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with chicken pAb to ankyrin 3, CH22125, dilution 1:2,000 in green, and costained with mouse mAb to FOX3/NeuN dilution 1:2,000 in red. The blue is Hoechst staining of nuclear DNA. The CH22125 antibody stains the axonal initial segments, while the FOX3/NeuN antibody reveals perikarya and nuclei of neurons.
Western blot analysis of different tissue and cell lysates using chicken pAb to ankyrin 3 dilution 1:3,000, in green. [1, 6] Protein standard, [2] Rat brain, [3] Cow cortex, [4] HEK293 cells, [5] COS-1 cells, [7] Rat cortex, [8] rat cortex membrane enriched fraction, [9] mouse cortex, [10] mouse cortex membrane enriched fraction.
Western blot analysis of different tissue and cell lysates using chicken pAb to ankyrin 3 dilution 1:3,000, in green. [1, 6] Protein standard, [2] Rat brain, [3] Cow cortex, [4] HEK293 cells, [5] COS-1 cells, [7] Rat cortex, [8] rat cortex membrane enriched fraction, [9] mouse cortex, [10] mouse cortex membrane enriched fraction.