Product Details
Catalog Number: CH22125
Applications: WB, IHC, IF
Type: Chicken IgY
Dilutions: IF: 1:500-1,000 IHC: 1:500-1,000 WB: 1:5,000-10,000
Immunogen: C-terminal 398 amino acids of human ankyrin 3 expressed in and purified in E. coli
Storage: Store at 4°C short term. Store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat, Primate, Bovine
Entrez: 288
UniProt: Q12955
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
100 ul of 1 mg/ml$295.00Add to Cart

Ankyrin 3, also known as Ankyrin G, is larger than the related Ankyrin 1 and Ankyrin 2.  Ankyrin 3 is expressed in the axon initial segment and the nodes of Ranvier in the nervous system. Antibodies against Ankyrin 3 thus can be useful in identifying the regions of axon initial segments and nodes of Ranvier. This antibody was made against the C-terminal 398 amino acid of human isotype 1, and works well on human and rat ankyrin 3 on western blots and section material. However, this antibody does not work on mouse extracts and tissues.

Images
Immunofluorescence

Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with chicken pAb to ankyrin 3, CH22125, dilution 1:2,000 in green, and costained with mouse mAb to FOX3/NeuN dilution 1:2,000 in red. The blue is Hoechst staining of nuclear DNA. The CH22125 antibody stains the axonal initial segments, while the FOX3/NeuN antibody reveals perikarya and nuclei of neurons.

Immunofluorescence

Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with chicken pAb to ankyrin 3, CH22125, dilution 1:2,000 in green, and costained with mouse mAb to FOX3/NeuN dilution 1:2,000 in red. The blue is Hoechst staining of nuclear DNA. The CH22125 antibody stains the axonal initial segments, while the FOX3/NeuN antibody reveals perikarya and nuclei of neurons.

Western Blot

Western blot analysis of different tissue and cell lysates using chicken pAb to ankyrin 3 dilution 1:3,000, in green. [1, 6] Protein standard, [2] Rat brain, [3] Cow cortex, [4] HEK293 cells, [5] COS-1 cells, [7] Rat cortex, [8] rat cortex membrane enriched fraction, [9] mouse cortex, [10] mouse cortex membrane enriched fraction.

Western Blot

Western blot analysis of different tissue and cell lysates using chicken pAb to ankyrin 3 dilution 1:3,000, in green. [1, 6] Protein standard, [2] Rat brain, [3] Cow cortex, [4] HEK293 cells, [5] COS-1 cells, [7] Rat cortex, [8] rat cortex membrane enriched fraction, [9] mouse cortex, [10] mouse cortex membrane enriched fraction.