Product Details
Catalog Number: CH22126
Applications: ICC, WB, IHC, IF
Type: Chicken IgY
Dilutions: Immunohistochemistry: 1:500 Immunofluorescent: 1:500 Immunohistochemistry: 1:500 Western Blot: 1:2,000
Immunogen: Recombinant full length human NSE expressed in and purified from E. coli.
Storage: Store at 4°C short term. Store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Whole serum
Species Reactivity: Human, Mouse, Rat
Entrez: 2026
UniProt: P09104
Downloads: Datasheet (pdf)
Product Sizes
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100 ul of serum$303.00Add to Cart

Neuron specific enolase (NSE) is an enzyme which catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and also the reverse reaction in gluconeogenesis. It is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or alternately as α, β and γ enolase. The three enolases are related in protein sequence, and have different cell type specific expression patterns, so that antibodies to them are useful cell type specific markers. NSE is also known as enolase 2 or γ enolase and is heavily expressed in neuronal cells. Enolase 1 is also known as α enolase and as non-neuronal enolase. The third enolase, enolase 3 or β enolase, is expressed in muscle cells. Perhaps not surprisingly, since neurons require a great deal of energy, they are very rich in glycolytic enzymes such a GAPDH and NSE. Antibodies to this protein are therefore useful to identify neuronal cell bodies, and also developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury, and elevated NSE levels in blood and tissues are seen associated with various kinds of neuroendocrine derived tumors.

Images
Immunofluorescent

Immunofluorescent analysis of a section of adult mouse cerebellar dentate nucleus stained with chicken pAb to neuron specific enolase (NSE), CH22126, dilution 1:3,000 in red. The section was costained with rabbit pAb to GFAP dilution 1:5,000 in green. The blue is Hoechst staining of nuclear DNA.

Immunofluorescent

Immunofluorescent analysis of a section of adult mouse cerebellar dentate nucleus stained with chicken pAb to neuron specific enolase (NSE), CH22126, dilution 1:3,000 in red. The section was costained with rabbit pAb to GFAP dilution 1:5,000 in green. The blue is Hoechst staining of nuclear DNA.

Western Blot

Western blot analysis of tissue and cell lysates using chicken pAb to neuron specific enolase (NSE), CH22126, dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] cow brain, and [5] SH-SY5Y cells. the strong band running at about 47kDa corresponds to the NSE protein.

Western Blot

Western blot analysis of tissue and cell lysates using chicken pAb to neuron specific enolase (NSE), CH22126, dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] cow brain, and [5] SH-SY5Y cells. the strong band running at about 47kDa corresponds to the NSE protein.