Product Details
Catalog Number: CH22127
Applications: ICC, WB, IHC, IF
Type: Chicken IgY
Dilutions: Immunofluorescence: 1:5,000-10,000; Immunohistochemistry: 1:5,000-10,000; Western Blot: 1:500-1,000
Immunogen: C-terminal peptide of rat ACIII, PAAFPNGSSVTLPHQVVDNP with a Cys added to the N-terminus to allow coupling to KLH.
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (polar packs)
Format A: liquid
Species Reactivity: Mouse, Rat
Entrez: 109
UniProt: O60266
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$299.00Add to Cart

Trimeric G-proteins are a large and variable family of membrane receptors. On binding their specific ligand they activate specific members of the family of trimeric G-proteins which in turn activate other signalling enzymes. Adenylate cyclases are one of these downstream enzyme families which are activated by the GTP bound GαS subunits of trimeric G-proteins. Adenylate cyclases are responsible for the production of the important “second messenger” signaling molecule cyclic-AMP which in turn activates the cAMP dependent protein kinase, which phosphorylates and regulates numerous other molecules. There are several different adenylate cyclase gene products, the type III adenylate cyclase being specifically localized in neuronal cilia. Much interest has focused on neuronal cilia since they express a variety of receptors and appear to function as a unique and previously not well understood sensory structure of neurons. For examples, the somatostatin 3 receptor, NPY2 receptor and melanin concentrating hormone receptor 1 are localized in neuronal cilia and the sonic hedgehog and Wnt signalling pathway act primarily through neuronal cilia. This antibody is an excellent marker of neuronal cilia in the brain and in cells in tissue culture and works in the same way as our rabbit polyclonal made against the same peptide.

This antibody was made against the C-terminal peptide of rat ACIII, works on mouse and human cells and is an excellent marker of neuronal cilia in the brain and in cells in tissue culture.

Images
Immunofluorescence

Immunofluorescence analysis of rat cortex section stained with chicken pAb to adenylate cyclase III, CH22127, dilution 1:10,000, in red and costained with mouse mAb to the myelin and oligodendrocyte marker CNP in green. The blue is Hoechst staining of nuclear DNA. The ACIII antibody reveals neuronal cilia while the CNP antibody stains oligodendrocytes and the myelin sheath around axons.

Immunofluorescence

Immunofluorescence analysis of rat cortex section stained with chicken pAb to adenylate cyclase III, CH22127, dilution 1:10,000, in red and costained with mouse mAb to the myelin and oligodendrocyte marker CNP in green. The blue is Hoechst staining of nuclear DNA. The ACIII antibody reveals neuronal cilia while the CNP antibody stains oligodendrocytes and the myelin sheath around axons.

Western Blot

Western blot analysis of different tissue lysates using chicken pAb to ACIII, CH22127, dilution 1:1,000, in green. On the left blot: [1] protein standard, [2] rat hippocampus, [3] mouse hippocampus, [4] mouse frontal cortex, and [5] cow frontal cortex. On the right blot: [6] protein standard, [7] non-transfected HEK293 cells, and [8] HEK293 cells transfected with DNA expressing Myc-DDK tagged full length human adenylate cyclase III from the appropriate cDNA (ACIII).

Western Blot

Western blot analysis of different tissue lysates using chicken pAb to ACIII, CH22127, dilution 1:1,000, in green. On the left blot: [1] protein standard, [2] rat hippocampus, [3] mouse hippocampus, [4] mouse frontal cortex, and [5] cow frontal cortex. On the right blot: [6] protein standard, [7] non-transfected HEK293 cells, and [8] HEK293 cells transfected with DNA expressing Myc-DDK tagged full length human adenylate cyclase III from the appropriate cDNA (ACIII).