Images
Immunofluorescence

Immunofluorescence analysis of rat cortex section stained with chicken pAb to adenylate cyclase III, CH22127, dilution 1:10,000, in red and costained with mouse mAb to the myelin and oligodendrocyte marker CNP in green. The blue is Hoechst staining of nuclear DNA. The ACIII antibody reveals neuronal cilia while the CNP antibody stains oligodendrocytes and the myelin sheath around axons.

Immunofluorescence

Immunofluorescence analysis of rat cortex section stained with chicken pAb to adenylate cyclase III, CH22127, dilution 1:10,000, in red and costained with mouse mAb to the myelin and oligodendrocyte marker CNP in green. The blue is Hoechst staining of nuclear DNA. The ACIII antibody reveals neuronal cilia while the CNP antibody stains oligodendrocytes and the myelin sheath around axons.

Western Blot

Western blot analysis of different tissue lysates using chicken pAb to ACIII, CH22127, dilution 1:1,000, in green. On the left blot: [1] protein standard, [2] rat hippocampus, [3] mouse hippocampus, [4] mouse frontal cortex, and [5] cow frontal cortex. On the right blot: [6] protein standard, [7] non-transfected HEK293 cells, and [8] HEK293 cells transfected with DNA expressing Myc-DDK tagged full length human adenylate cyclase III from the appropriate cDNA (ACIII).

Western Blot

Western blot analysis of different tissue lysates using chicken pAb to ACIII, CH22127, dilution 1:1,000, in green. On the left blot: [1] protein standard, [2] rat hippocampus, [3] mouse hippocampus, [4] mouse frontal cortex, and [5] cow frontal cortex. On the right blot: [6] protein standard, [7] non-transfected HEK293 cells, and [8] HEK293 cells transfected with DNA expressing Myc-DDK tagged full length human adenylate cyclase III from the appropriate cDNA (ACIII).

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