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Immunofluorescent analysis of rat cerebellum section stained with chicken pAb to CNP, CH22128, dilution 1:2,000 in green and costained with rabbit pAb to NF-H in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies.
Immunofluorescent analysis of rat cerebellum section stained with chicken pAb to CNP, CH22128, dilution 1:2,000 in green and costained with rabbit pAb to NF-H in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies.
Western blot analysis of spinal cord tissue lysates using chicken pAb to CNP, CH22128, dilution 1:5,000, in red: [1] protein standard (red), [2] mouse, [3] rat, and [4] cow spinal cord. A doublet of bands at 46 and 48kDa correspond to isotypes of the CNP protein. The blot was simultaneously probed with mouse mAb to α-internexin in green. Major bands in the 64-66 kDa range corresponds to α-internexin.
Western blot analysis of spinal cord tissue lysates using chicken pAb to CNP, CH22128, dilution 1:5,000, in red: [1] protein standard (red), [2] mouse, [3] rat, and [4] cow spinal cord. A doublet of bands at 46 and 48kDa correspond to isotypes of the CNP protein. The blot was simultaneously probed with mouse mAb to α-internexin in green. Major bands in the 64-66 kDa range corresponds to α-internexin.