Product Details
Catalog Number: CH22128
Applications: ICC, WB, IHC, IF
Type: Chicken IgY
Dilutions: Immunofluorescence: 1:2,000; Immunohistochemistry: 1:2000; Western Blot: 1:5,000-10,000
Immunogen: Full length human recombinant protein
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (polar packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat, Bovine, Porcine, Equine
Entrez: 1267
UniProt: P09543
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
100 ul$275.00Add to Cart

The 2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNP), is an enzyme which catalyzes the hydrolysis of 2′, 3′-cyclic nucleotides to 2′-nucleotides. These cyclic nucleotides are structurally different from the better known and studied 3′-5′-cyclic nucleotides of which the best known example is cyclic AMP. CNP has two isoforms, CNPase 1 (46kDa) and CNPase 2 (48kDa), which are encoded separately by different promoters of the same gene. These enzymes are present in very high levels in brain and peripheral nerve, makes up 4% of total CNS myelin protein. They are found almost exclusively in oligodendrocytes and Schwann cells, appearing early in oligodendrocyte development, earlier than most other myelin specific proteins. Antibodies to CNP have been very useful as a marker for these particular cell types. CNP is thought to play a critical role in the events leading up to myelination, for the oligodendrocytes overexpressing CNP appear to mature earlier in development, resulting in earlier maximum gene expression for myelin basic proteins. It has been reported that CNP is also associated with microtubules in brain tissue and may promote microtubule assembly. CNP can link tubulin to cellular membranes, and may regulate cytoplasmic microtubule distribution. In various diseases, neurological mutants, and in experimental conditions in which myelin is reduced, CNP levels may also be severely reduced. Decreased brain levels of CNP have also been reported in Down syndrome and Alzheimer’s disease.

This antibody was made against the full length recombinant form of human CNP expressed in and purified from E. Coli, and the antibody can be used to identify myelinating cells in cell culture and in sections and to trace axonal projections in sectioned material.

Images
Immunofluorescence

Immunofluorescent analysis of rat cerebellum section stained with chicken pAb to CNP, CH22128, dilution 1:2,000 in green and costained with rabbit pAb to NF-H in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies.

Immunofluorescence

Immunofluorescent analysis of rat cerebellum section stained with chicken pAb to CNP, CH22128, dilution 1:2,000 in green and costained with rabbit pAb to NF-H in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies.

Western Blot

Western blot analysis of spinal cord tissue lysates using chicken pAb to CNP, CH22128, dilution 1:5,000, in red: [1] protein standard (red), [2] mouse, [3] rat, and [4] cow spinal cord. A doublet of bands at 46 and 48kDa correspond to isotypes of the CNP protein. The blot was simultaneously probed with mouse mAb to α-internexin in green. Major bands in the 64-66 kDa range corresponds to α-internexin.

Western Blot

Western blot analysis of spinal cord tissue lysates using chicken pAb to CNP, CH22128, dilution 1:5,000, in red: [1] protein standard (red), [2] mouse, [3] rat, and [4] cow spinal cord. A doublet of bands at 46 and 48kDa correspond to isotypes of the CNP protein. The blot was simultaneously probed with mouse mAb to α-internexin in green. Major bands in the 64-66 kDa range corresponds to α-internexin.