Product Details
Catalog Number: CH22129
Applications: ICC, WB, IHC, IF
Type: Chicken IgY
Dilutions: Immunofluorescence: 1:2,000-5,000; Immunohistochemistry: 1:2,000-5,000; Western Blot: 1:2,000-5,000
Immunogen: Full length human fibrillarin expressed in and purified from E. coli.
Storage: Store at 4°C.
Shipping: Frozen (polar packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat, Dog, Equine
Entrez: 2091
UniProt: P22087
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$275.00Add to Cart

Fibrillarin is a highly conserved component of a nucleolar small ribonucleoprotein complex in mammals, involved in the processing of ribosomal RNA during ribosomal biogenesis. The protein runs at ~35kDa on SDS-PAGE and is very rich in basic amino acids having a PI of 9.8. Fibrillarin was originally identified in humans since autoantibodies staining nucleoli were seen in some patients with the autoimmune disease scleroderma. Subsequently the protein fibrillarin was found to be the human homologue of Nop1p, a Saccharomyces cerevisiae nucleolar protein, the two proteins being 67% identical. We have generated an alignment of the sequences of fibrillarin and homologues downloadable from here. The fibrillarin molecule consists of an N-terminal glycine and arginine rich region followed by a highly conserved globular domain. Embryonic knockout of the fibrillarin gene in mice is lethal, suggesting fundamental importance of this protein. Autoantibodies to fibrillarin are also seen in patients with the autoimmune disease systemic sclerocis.

Images
Immunofluorescene

Confocal immunofluorescent analysis of HeLa cells stained with chicken pAb to fibrillarin, CH22129, dilution 1:10,000 in green and costained with mouse mAb to vimentin in red. The blue signal is DAPI staining of nuclear DNA. The fibrillarin antibody stains nucleoli while the vimentin antibody binds to cytoplasmic intermediate filaments.

Immunofluorescene

Confocal immunofluorescent analysis of HeLa cells stained with chicken pAb to fibrillarin, CH22129, dilution 1:10,000 in green and costained with mouse mAb to vimentin in red. The blue signal is DAPI staining of nuclear DNA. The fibrillarin antibody stains nucleoli while the vimentin antibody binds to cytoplasmic intermediate filaments.

Western Blot

Western blot analysis of different cell lysates using chicken pAb to fibrillarin, CH22129, dilution 1:5,000, in green: [1] protein standard (red), [2] NIH-3T3, [3] HEK293, [4] HeLa, [5] SH-SY5Y, and [6] C6 cells. The single strong band at ~35kDa correspond to the fibrillarin protein.

Western Blot

Western blot analysis of different cell lysates using chicken pAb to fibrillarin, CH22129, dilution 1:5,000, in green: [1] protein standard (red), [2] NIH-3T3, [3] HEK293, [4] HeLa, [5] SH-SY5Y, and [6] C6 cells. The single strong band at ~35kDa correspond to the fibrillarin protein.