Images
Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with chicken pAb to HSP27, CH22133, dilution 1:1,000 in green, and costained with mouse mAb to HSP60 in red. Blue is DAPI staining of nuclear DNA. The CPCA-HSP27 antibody produces strong cytoplasmic staining, while the HSP60 antibody specifically labels mitochondria.

Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with chicken pAb to HSP27, CH22133, dilution 1:1,000 in green, and costained with mouse mAb to HSP60 in red. Blue is DAPI staining of nuclear DNA. The CPCA-HSP27 antibody produces strong cytoplasmic staining, while the HSP60 antibody specifically labels mitochondria.

Western Blot

Western blot analysis of whole cell lysates using chicken pAb to HSP27, CH22133, dilution 1:2,000, in red. [1] protein standard (red), [2] NIH-3T3 , [3] C6, [4] HeLa, [5] HEK293, [6] SH-SY5Y, and [7] COS-1 cells. Strong single band at 27kDa corresponds to the HSP27 protein strongly expressed in human cell lines.

Western Blot

Western blot analysis of whole cell lysates using chicken pAb to HSP27, CH22133, dilution 1:2,000, in red. [1] protein standard (red), [2] NIH-3T3 , [3] C6, [4] HeLa, [5] HEK293, [6] SH-SY5Y, and [7] COS-1 cells. Strong single band at 27kDa corresponds to the HSP27 protein strongly expressed in human cell lines.

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