Product Details
Catalog Number: CH22134
Applications: ICC, WB, IHC, IF
Type: Chicken IgY
Dilutions: Immunofluorescence: 1:2,000-5,000; Immunohistochemistry: 1:2,000-5,000; Western Blot: 1:1,000-5,000
Immunogen: Peptide identical to part of the C-terminal of human IBA1 coupled to KLH
Storage: Store at 4°C.
Shipping: Frozen (polar packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat
Entrez: 199
UniProt: P55008
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$275.00Add to Cart

IBA1 is an acronyn for “ionized Calcium binding adapter molecule 1”, and the protein is also known as AIF1 for “allograft inflammatory factor 1”. AIF1 was originally identified, cloned and sequenced as a protein heavily upregulated in an animal model of graft rejection. Suitable IBA1 antibodies are widely used to identify microglial cells in sections and tissues. In tissue samples from which they have not been washed out by perfusion, lymphocytes within blood vessels are also IBA1 positive. Microglia are the immunocompetent cells of the CNS and are extremely important in responses to injury and disease. Microglial are small but very active cells which constantly send processes probing their neighborhood and which alter morphology and are induced to divide following a variety of CNS compromises. Many important and highly cited papers have made use of IBA1 antibodies as markers of microglia.

This antibody was made against the C-terminal peptide of human IBA1 coupled to keyhole limpet hemocyanin. It works well on western blots, on cells cultures and sectioned material.

Images
Immunofluorescence

Rat spinal cord section, stained with CH22134, dilution 1:1,000, in red. Microglia are very small cells with fine processes spreading in three dimensions and so are best visualized in a confocal Z stack. Nuclear DNA is shown with DAPI stain in blue.

Immunofluorescence

Rat spinal cord section, stained with CH22134, dilution 1:1,000, in red. Microglia are very small cells with fine processes spreading in three dimensions and so are best visualized in a confocal Z stack. Nuclear DNA is shown with DAPI stain in blue.

Western Blot

Western blot analysis of chicken pAb to IBA1, CH22134, dilution 1:1,000 in green: [1] protein standard (red) and [2] rat spleen crude homogenate. The band at about 15kDa mark corresponds to IBA1 protein. IBA1 is a relatively minor protein of brain and is much more abundant in spleen, so the 15kDa band is less obvious in CNS lysates.

Western Blot

Western blot analysis of chicken pAb to IBA1, CH22134, dilution 1:1,000 in green: [1] protein standard (red) and [2] rat spleen crude homogenate. The band at about 15kDa mark corresponds to IBA1 protein. IBA1 is a relatively minor protein of brain and is much more abundant in spleen, so the 15kDa band is less obvious in CNS lysates.