Product Details
Catalog Number: CH22135
Applications: ICC, WB, IF
Type: Chicken IgY
Dilutions: Immunofluorescence: 1:1,000-5,000; Western Blot: 1:5,000
Immunogen: Mixture of recombinant human Ki67 constructs expressing the N-terminal region, amino acids 1-300 and an internal region, amino acids 1111-1490
Storage: Store at 4°C.
Shipping: Frozen (polar packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat
Entrez: 4288
UniProt: P46013
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$275.00Add to Cart

The Ki67 protein was first discovered when researchers attempted to generate cancer cell specific monoclonal antibodies by injecting mice with nuclear preparations from Hodgkin’s lymphoma cells. They obtained a monoclonal antibody which recognized two large proteins of apparent molecular weight 345kDa and 395kDa. The presence of the Ki67 protein is frequently used as an indicator of cell proliferation and its level of expression is one of the most reliable biomarkers of proliferative status of cancer cells. Much research shows a correlation between Ki67 protein level and prognosis in cancer patients, when high Ki67 levels being associated with poorer outcomes.

Images
Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with chicken pAb to Ki67, CH22135, dilution 1:2,000 in red, and mouse mAb to β-tubulin in green. The blue is DAPI staining of nuclear DNA. The CPCA-Ki67 antibody detects Ki67 protein predominantly expressed in nucleoli of cells in interphase and in mitotic cells Ki67 forms a coat around condensed chromosomes. Ki67 is not detected in cells in the quiescent G0 state.

Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with chicken pAb to Ki67, CH22135, dilution 1:2,000 in red, and mouse mAb to β-tubulin in green. The blue is DAPI staining of nuclear DNA. The CPCA-Ki67 antibody detects Ki67 protein predominantly expressed in nucleoli of cells in interphase and in mitotic cells Ki67 forms a coat around condensed chromosomes. Ki67 is not detected in cells in the quiescent G0 state.

Western Blot

Western blot analysis of equal amounts of cell lysates probed with chicken pAb to Ki67, CH22135, dilution 1:5,000, in green: [1] protein standard (red), [2] rapidly dividing HeLa cell cultures, [3] rapidly dividing HEK293 cell cultures. Strong double bands above 250kDa correspond to the two major Ki67 isoforms of molecular weight 345kDa and 395kDa.

Western Blot

Western blot analysis of equal amounts of cell lysates probed with chicken pAb to Ki67, CH22135, dilution 1:5,000, in green: [1] protein standard (red), [2] rapidly dividing HeLa cell cultures, [3] rapidly dividing HEK293 cell cultures. Strong double bands above 250kDa correspond to the two major Ki67 isoforms of molecular weight 345kDa and 395kDa.