Product Details
Catalog Number: CH22136
Applications: ICC, WB, IF
Type: Chicken IgY
Dilutions: Immunofluorescence: 1:1,000; Western Blot: 1:2,000
Immunogen: Full length recombinant human lamin A protein expressed in and purified from E. coli.
Storage: Store at 4°C.
Shipping: Frozen (polar packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat, Primate, Dog, Equine
Entrez: 4000
UniProt: P02545
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$275.00Add to Cart

Lamin A and lamin C are members of the intermediate filament protein family and are located in the nucleus where they function as skeletal components of the inner nuclear membrane. The two proteins are generated by alternate transcription from the single LMNA gene. Lamin A has a molecular weight of about 74kDa while lamin C is 65kDa. The lamin A protein includes a C-terminal segment of 98 amino acids missing from lamin C, while lamin C has a unique C-terminal 6 amino acid peptide not present in lamin A. As a result antibodies raised against lamin A are almost certain bind to lamin C. During cell division the nuclear lamina breaks down and lamin A/C containing filaments depolymerize, this being regulated by phosphorylation by cyclin dependent protein kinase 1. Mutations in the lamin A/C gene are associated with several serious human diseases.

This antibody was raised against full length recombinant human lamin A, binds human lamin C, and works with the rodent protein also.

Images
Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with chicken pAb to lamin A/C, CH22136, dilution 1:2,000 in red, and costained with mouse mAb to actin in green. The blue is Hoechst staining of nuclear DNA. The antibody specifically labels the nuclear lamina, while the actin antibody stains the submembranous actin-rich cytoskeleton, stress fibers and bundles of actin associated with cell adhesion sites.

Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with chicken pAb to lamin A/C, CH22136, dilution 1:2,000 in red, and costained with mouse mAb to actin in green. The blue is Hoechst staining of nuclear DNA. The antibody specifically labels the nuclear lamina, while the actin antibody stains the submembranous actin-rich cytoskeleton, stress fibers and bundles of actin associated with cell adhesion sites.

Western Blot

Western blot analysis of cytosolic or nuclear enriched fractions of cell lines probed with chicken pAb to lamin A/C, CH22136, dilution 1:1,000 in green: [1] protein standard (red), [2] HeLa cytosol, [3] HeLa nuclear, [4] NIH-3T3 cytosol, and [5] NIH-3T3 nuclear fractions. Two strong bands at 65kDa and 74kDa correspond to lamin A and lamin C proteins respectively, detected exclusively in the nuclear fractions.

Western Blot

Western blot analysis of cytosolic or nuclear enriched fractions of cell lines probed with chicken pAb to lamin A/C, CH22136, dilution 1:1,000 in green: [1] protein standard (red), [2] HeLa cytosol, [3] HeLa nuclear, [4] NIH-3T3 cytosol, and [5] NIH-3T3 nuclear fractions. Two strong bands at 65kDa and 74kDa correspond to lamin A and lamin C proteins respectively, detected exclusively in the nuclear fractions.