Images
Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with chicken pAb to lamin A/C, CH22136, dilution 1:2,000 in red, and costained with mouse mAb to actin in green. The blue is Hoechst staining of nuclear DNA. The antibody specifically labels the nuclear lamina, while the actin antibody stains the submembranous actin-rich cytoskeleton, stress fibers and bundles of actin associated with cell adhesion sites.

Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with chicken pAb to lamin A/C, CH22136, dilution 1:2,000 in red, and costained with mouse mAb to actin in green. The blue is Hoechst staining of nuclear DNA. The antibody specifically labels the nuclear lamina, while the actin antibody stains the submembranous actin-rich cytoskeleton, stress fibers and bundles of actin associated with cell adhesion sites.

Western Blot

Western blot analysis of cytosolic or nuclear enriched fractions of cell lines probed with chicken pAb to lamin A/C, CH22136, dilution 1:1,000 in green: [1] protein standard (red), [2] HeLa cytosol, [3] HeLa nuclear, [4] NIH-3T3 cytosol, and [5] NIH-3T3 nuclear fractions. Two strong bands at 65kDa and 74kDa correspond to lamin A and lamin C proteins respectively, detected exclusively in the nuclear fractions.

Western Blot

Western blot analysis of cytosolic or nuclear enriched fractions of cell lines probed with chicken pAb to lamin A/C, CH22136, dilution 1:1,000 in green: [1] protein standard (red), [2] HeLa cytosol, [3] HeLa nuclear, [4] NIH-3T3 cytosol, and [5] NIH-3T3 nuclear fractions. Two strong bands at 65kDa and 74kDa correspond to lamin A and lamin C proteins respectively, detected exclusively in the nuclear fractions.

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