Product Details
Catalog Number: CH22138
Applications: ICC, WB, IHC, IF
Type: Chicken IgY
Dilutions: Immunofluorescence: 1:1,000-2,000; Immunohistochemistry: 1:1,000-2,000; Western Blot: 1:10,000-20,000
Immunogen: Full length recombinant human MeCP2 expressed in and purified from E. coli.
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (polar packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat, Primate
Entrez: 4204
UniProt: P51608
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$275.00Add to Cart

Methyl-CpG Binding Protein 2 (MeCP2) is widely expressed in tissues and particularly heavily in neurons. It is a nuclear protein which was found to bind methylated cytosines in CpG islands in DNA, but which may also bind hydroxymethyl cytosine residues. This antibody was made against full length recombinant human MeCP2 expressed in and purified from E. coli and can be used to identify neurons transiently expressing high levels of this nuclear protein.

Images
Immunofluorescence

Immunofluorescent analysis of rat olfactory bulb section stained with chicken pAb to MeCP2, CH22138, dilution 1:2,000 in red and costained with mouse mAb to α-synuclein in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with the above antibodies.

Immunofluorescence

Immunofluorescent analysis of rat olfactory bulb section stained with chicken pAb to MeCP2, CH22138, dilution 1:2,000 in red and costained with mouse mAb to α-synuclein in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with the above antibodies.

Western Blot

Western blot analysis of tissue and cell lysates using chicken pAb to MeCP2, CH22138, dilution 1:20,000 in green: [1] protein standard (red), [2] rat whole brain, [3] nuclear fraction of rat brain, [4] mouse whole brain, [5] nuclear fraction of mouse brain lysate, [6] C6 cell lysate, and [7] SH-SY5Y cell lysate. The strong band at about 75kDa corresponds to the MeCP2 protein.

Western Blot

Western blot analysis of tissue and cell lysates using chicken pAb to MeCP2, CH22138, dilution 1:20,000 in green: [1] protein standard (red), [2] rat whole brain, [3] nuclear fraction of rat brain, [4] mouse whole brain, [5] nuclear fraction of mouse brain lysate, [6] C6 cell lysate, and [7] SH-SY5Y cell lysate. The strong band at about 75kDa corresponds to the MeCP2 protein.