Product Details
Catalog Number: CH22139
Applications: ICC, WB, IHC, IF
Type: Chicken IgY
Dilutions: Immunofluorescence: 1:1,000; Immunohistochemistry: 1:1,000; Western Blot: 1:2,000
Immunogen: Full length recombinant human protein expressed in and purified from E. coli
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (polar packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat, Bovine, Porcine, Equine
Entrez: 6622
UniProt: P37840
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$299.00Add to Cart

α-synuclein is a member of the synuclein protein family, the other two members being β and γ synuclein, each protein being coded for by a distinct but related gene. α-synuclein was originally isolated as a major synaptic vesicle associated protein from the electric organ of the fish Torpedo, and direct homologues of α-synuclein are found in all vertebrates. Later work connected α-synuclein expression with several human brain pathologies, so that it is a major component of the Lewy bodies of Parkinson’s disease. Point mutations of α-synuclein proved to be causative of some forms of familial Parkinson’s disease. α-synuclein is also found in the Lewy bodies of patients with diffuse Lewy body disease and inclusions in glial cells in the brains of patients with multiple system atrophy and amyotrophic lateral sclerosis. α-synuclein is normally heavily expressed in brain and appears to be localized primarily to presynaptic regions, though not with a typical synaptic vesicle distribution pattern.

This antibody recognizes full length human and rodent α-synuclein specifically both in western blots and in immunocytochemical experiments and is a good marker of synapses on transgenic mice.

Images
Immunofluorescence

Immunofluorescent analysis of a section of rat cerebellum stained with chicken pAb to α-synuclein, CH22139, dilution 1:3,000 in red, and costained with rabbit pAb to GFAP in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies.

Immunofluorescence

Immunofluorescent analysis of a section of rat cerebellum stained with chicken pAb to α-synuclein, CH22139, dilution 1:3,000 in red, and costained with rabbit pAb to GFAP in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies.

Western Blot

Western blot analysis of different tissue lysates using chicken pAb to α-synuclein, CH22139, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at about 15kDa corresponds to the α-synuclein protein in brain extracts, which are rich in synapses, while a weaker band is seen in spinal cord extracts where synapses are a more minor component.

Western Blot

Western blot analysis of different tissue lysates using chicken pAb to α-synuclein, CH22139, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at about 15kDa corresponds to the α-synuclein protein in brain extracts, which are rich in synapses, while a weaker band is seen in spinal cord extracts where synapses are a more minor component.