Product Details
Catalog Number: CH22140
Applications: ICC, WB, IHC, IF
Type: Chicken IgY
Dilutions: Immunofluorescence: 1:500-1,000; Immunohistochemistry: 1:500-1,000; Western Blot: 1:2,000-5,000
Immunogen: Recombinant full length human UCHL1 expressed in and purified from E. coli.
Storage: Store at 4°C.
Shipping: Frozen (polar packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat, Bovine, Porcine, Equine
Entrez: 7345
UniProt: P09936
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$275.00Add to Cart

Ubiquitin C-terminal hydrolase 1 (UCHL1) is an extremely abundant protein of brain, where it is localized only in neurons. It was originally named PGP9.5 and discovered as a major protein spot on 2D gels of brain extracts which was absent on similar gels of other tissues. Later it was found that the PGP9.5 protein was an enzyme which could cleave ubiquitin monomers from ubiquitin conjugates and polyubiquitin chains, resulting in recycling of ubiquitin monomers and the renaming of PGP9.5 to UCHL1 to reflect this enzymatic activity. UCHL1 is an essential enzyme and defects in UCHL1 protein expression are involved in Parkinson’s disease (PD) and other more serious disease states. In addition, UCHL1 may be released into cerebrospinal fluid (CSF) and blood following CNS damage and disease resulting in neuronal loss.

This antibody was made against full length recombinant human UCHL1 expressed in and purified from E. coli and can be used to identify neurons and their processes in culture or in sections. The antibody works cleanly on appropriate lysates of cell and tissues. Considerable interest has been focused on the detection of UCHL1 in the blood and CSF of patients with traumatic injuries to the brain or spinal cord. This antibody has been widely used as both a capture and a detection reagent in ELISA type assays for measuring UCHL1 levels in blood and CSF samples.

Images
Immunofluorescence

Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with chicken pAb to UCHL1, CH22140, dilution 1:500 in red, and costained with mouse mAb to vimentin in green. The blue is Hoechst staining of nuclear DNA. The UCHL1 antibody produces strong staining of the cell body and dendrites in neurons. The vimentin antibody stains intermediate filaments in fibroblastic and developing glial cells.

Immunofluorescence

Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with chicken pAb to UCHL1, CH22140, dilution 1:500 in red, and costained with mouse mAb to vimentin in green. The blue is Hoechst staining of nuclear DNA. The UCHL1 antibody produces strong staining of the cell body and dendrites in neurons. The vimentin antibody stains intermediate filaments in fibroblastic and developing glial cells.

Western Blot

Western blot analysis of equal amounts of different tissue and cell lysates using chicken pAb to UCHL1, CH22140, dilution 1:2,000 in green, and mouse mAb to Actin in red: [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa and [7] SH-SY5Y cells. The single band at 24 kDa mark corresponds to UCHL1 protein which is detectable in CNS extracts and lysates of cells with neuronal properties.

Western Blot

Western blot analysis of equal amounts of different tissue and cell lysates using chicken pAb to UCHL1, CH22140, dilution 1:2,000 in green, and mouse mAb to Actin in red: [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa and [7] SH-SY5Y cells. The single band at 24 kDa mark corresponds to UCHL1 protein which is detectable in CNS extracts and lysates of cells with neuronal properties.