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Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with chicken pAb to UCHL1, CH22140, dilution 1:500 in red, and costained with mouse mAb to vimentin in green. The blue is Hoechst staining of nuclear DNA. The UCHL1 antibody produces strong staining of the cell body and dendrites in neurons. The vimentin antibody stains intermediate filaments in fibroblastic and developing glial cells.
Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with chicken pAb to UCHL1, CH22140, dilution 1:500 in red, and costained with mouse mAb to vimentin in green. The blue is Hoechst staining of nuclear DNA. The UCHL1 antibody produces strong staining of the cell body and dendrites in neurons. The vimentin antibody stains intermediate filaments in fibroblastic and developing glial cells.
Western blot analysis of equal amounts of different tissue and cell lysates using chicken pAb to UCHL1, CH22140, dilution 1:2,000 in green, and mouse mAb to Actin in red: [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa and [7] SH-SY5Y cells. The single band at 24 kDa mark corresponds to UCHL1 protein which is detectable in CNS extracts and lysates of cells with neuronal properties.
Western blot analysis of equal amounts of different tissue and cell lysates using chicken pAb to UCHL1, CH22140, dilution 1:2,000 in green, and mouse mAb to Actin in red: [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa and [7] SH-SY5Y cells. The single band at 24 kDa mark corresponds to UCHL1 protein which is detectable in CNS extracts and lysates of cells with neuronal properties.