GFP expressing human umbilical vein endothelial cells were isolated from normal human umbilical vein and transfected with GFP-lentiviral particles at passage 1. Puromycin resistant GFP-HUVECs are passage 3 and are shipped frozen or in proliferating culture with a confluency of >90 %*. ENDO-Growth Media (MED001) is recommended for culture. Cells have an average additional population doubling levels >18 when cultured.
Characterization of the cells
HUVECs are negative for HIV-1, HBV, HCV, and mycoplasma.
*cells are shipped at 90% confluence. However, confluence can be lost during shipping. Please allow for 1-4 days to reach 90% confluence again.