Product Details
Catalog Number: GF01-T25
Applications: Cell Assays
Type: Primary Neurons
Storage: Keep frozen in liquid nitrogen until plating
Shipping: Frozen (Dry Ice/Liquid Nitrogen)
Species Reactivity: Human
Downloads: Datasheet (pdf)
Product Sizes
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500,000+ Cells in T25 flask$759.00Add to Cart

GFP expressing human umbilical vein endothelial cells were isolated from normal human umbilical vein and transfected with GFP-lentiviral particles at passage 1. Puromycin resistant GFP-HUVECs are passage 3 and are shipped frozen or in proliferating culture with a confluency of >90 %*. ENDO-Growth Media (MED001) is recommended for culture. Cells have an average additional population doubling levels >18 when cultured. 

Characterization of the cells
  • Cytoplasmic VWF / Factor VIII: >95% positive by immunofluorescence
  • Cytoplasmic uptake of Di-I-Ac-LDL: >95% positive by immunofluorescence
  • Cytoplasmic PECAM1 >95% positive by immunofluorescence

HUVECs are negative for HIV-1, HBV, HCV, and mycoplasma.

*cells are shipped at 90% confluence. However, confluence can be lost during shipping. Please allow for 1-4 days to reach 90% confluence again. 

Images

IVSWT-mediated HUVEC vasculogenesis. (A) Overview of the treatment setup for stimulation of EC/ASC co-cultures in fibrin. (B) Fluorescent images of non-treated versus treated LEC and HUVEC networks on day 7. (C) Quantifications of HUVEC networks. IVSWT increased the number of junctions, tubules and the total tubule length. The mean tubule length was decreased. P-values: *** ≤0.01, ** ≤0.1, * ≤0.5. doi:10.1371/journal.pone.0114806.g002.

IVSWT-mediated HUVEC vasculogenesis. (A) Overview of the treatment setup for stimulation of EC/ASC co-cultures in fibrin. (B) Fluorescent images of non-treated versus treated LEC and HUVEC networks on day 7. (C) Quantifications of HUVEC networks. IVSWT increased the number of junctions, tubules and the total tubule length. The mean tubule length was decreased. P-values: *** ≤0.01, ** ≤0.1, * ≤0.5. doi:10.1371/journal.pone.0114806.g002.