The histochemical antibody for serotonin is generated in a goat against serotonin conjugated to bovine serum albumin with paraformaldehyde.
The antibody has a proven 4+ indirect immunofluorescence staining at a 1/400 - 1/800 dilution and a 4+ biotin-streptavidin/HRP staining at a 1/4000 - 1/10,000 dilution in rat hypothalamus and spinal cord.
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- Weifang Rong, Ting Gao, Li Dong, Jiahong Qian, Xiaowei Ding, Yi Zheng, Meimei Wu, Li Meng, Yingfu Jiao, Po Gao, Ping Luo, Guohua Zhang, Changhao Wu, and Xueyin Shi. (2021). G-Protein-Coupled Estrogen Receptor (GPER) in the Rostral Ventromedial Medulla Is Essential for Mobilizing Descending Inhibition of Itch. Journal of Neuroscience, 41(37), 7727-7741. doi: 10.1523/JNEUROSCI.2592-20.2021
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