Product Details
Catalog Number: GT22101
Applications: WB, IHC, IF
Type: Goat IgG
Dilutions: IF: 1:5,000 IHC: 1:5,000 WB: 1:1,000-5,000
Immunogen: Recombinant Aequoria coerulescens GFP purified from E. coli
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (polar packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Not Applicable
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$325.00Add to Cart

GFP (green fluorescent protein) is 27kDa protein; which was original isolated from the jellyfish (Aequoria victoria). This antibody was made against a recombinant GFP, from the Aequoria species, referred to as AcGFP. This antibody can be used to verify the expression, size and stability of both AcGFP and eGFP fusion proteins in western blotting experiments and to amplify GFP signals in tissues of transgenic animals. 

Images

Immunofluorescent analysis of transfected HEK293 cells with a GFP-construct in green stained with goat pAb to GFP, GT22101, dilution 1:5,000 in red. The blue is Hoechst staining of nuclear DNA. The GT22101 antibody reveals GFP protein expressed only in transfected cells, as a result transfected cells are appeared express both red and green signals and so appear an orange-golden color. Untransfected cells show neither signal.

Immunofluorescent analysis of transfected HEK293 cells with a GFP-construct in green stained with goat pAb to GFP, GT22101, dilution 1:5,000 in red. The blue is Hoechst staining of nuclear DNA. The GT22101 antibody reveals GFP protein expressed only in transfected cells, as a result transfected cells are appeared express both red and green signals and so appear an orange-golden color. Untransfected cells show neither signal.