Product Details
Catalog Number: GT22103
Applications: ICC, WB, IHC, IF
Type: Goat IgG
Dilutions: IF, ICC, IHC: 1:2,000; WB: 1:5,000
Immunogen: Recombinant Human vimentin expressed in and purified in E. coli
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (polar packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Mouse, Rat, Bovine, Porcine, Equine
Entrez: 7431
UniProt: P08670
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$295.00Add to Cart

Immunohistochemical staining for Vimentin is also characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors.

Vimentin protein is found in the eye lens and cornea, and found in mesenchymal tissues in adult mammals. In the CNS, it is found in endothelia cells and developing neurons, developing and some mature astrocytes, microglia, mature Bergmann glia and ependymal. Vimentin antibodies are useful in studying stem cells.  

Images

Immunofluorescent analysis of HeLa cell culture stained with goat pAb to vimentin, GT22103, dilution 1:500 in green, and costained with mouse mAb to actin, MO22125 dilution 1:500 in red. Blue is Hoechst staining of nuclear DNA.

Immunofluorescent analysis of HeLa cell culture stained with goat pAb to vimentin, GT22103, dilution 1:500 in green, and costained with mouse mAb to actin, MO22125 dilution 1:500 in red. Blue is Hoechst staining of nuclear DNA.