Product Details
Catalog Number: GT22104
Applications: ICC, WB, IF
Type: Goat IgG
Dilutions: Immunofluorescent: 1:2,000-5,000 Immunocytochemistry: 1:2,000-5,000 Western Blot: 1:5,000-10,000
Immunogen: Purified myelin basic protein isolated from bovine brain
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Mouse, Rat, Bovine, Porcine
Entrez: 4155
UniProt: P02686
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
100 ul$303.00Add to Cart

Myelin Basic Protein (MBP) is one of the major proteins of the myelin sheath surrounding axons in the nervous system. Since it is of relatively low molecular weight and high abundance the protein sequence was determined from purified protein over 30 years ago. The protein is made by oligodendrocytes in the central and nervous system, so antibodies to MBP are good markers of this cell type. In the peripheral nervous system MBP is expressed by myelinating Schwann cells so this antibody can be used to identify these cells in culture or sections. In the central nervous system four different forms of the protein made by alternate transcription from a single gene, the protein products having molecular weights of 21.5, 20.5, 18.5, and 17.2kDa in humans.

Images
Immunofluorescence

: Immunofluorescent analysis of mouse hippocampus section stained with goat pAb to myelin basic protein (MBP), GT22104, dilution 1:5,000 in red, and costained with rabbit pAb to GFAP, dilution 1:5,000 in green.

Immunofluorescence

: Immunofluorescent analysis of mouse hippocampus section stained with goat pAb to myelin basic protein (MBP), GT22104, dilution 1:5,000 in red, and costained with rabbit pAb to GFAP, dilution 1:5,000 in green.

Western Blot

Western blot analysis of different tissue lysates using goat pAb to myelin basic protein (MBP), GT22104, dilution 1:5,000 in green: [1] protein standard (red), [2] rat cerebellum, [3] mouse cerebellum, [4] cow midbrain and [5] pig midbrain.

Western Blot

Western blot analysis of different tissue lysates using goat pAb to myelin basic protein (MBP), GT22104, dilution 1:5,000 in green: [1] protein standard (red), [2] rat cerebellum, [3] mouse cerebellum, [4] cow midbrain and [5] pig midbrain.