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Immunofluorescent analysis of a section of mouse striatum stained with goat pAb to CNP, GT22105, dilution 1:3,000 in green, and costained with rabbit pAb to NF-L in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, the brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies.
Immunofluorescent analysis of a section of mouse striatum stained with goat pAb to CNP, GT22105, dilution 1:3,000 in green, and costained with rabbit pAb to NF-L in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, the brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies.
Western blot analysis of different tissue lysates using goat pAb to CNP, GT22105, dilution 1:5,000, in green: [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] cow spinal cord, and [7] pig spinal cord. Bands at 46kDa and 48kDa mark correspond to the two major isotypes of CNP protein.
Western blot analysis of different tissue lysates using goat pAb to CNP, GT22105, dilution 1:5,000, in green: [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] cow spinal cord, and [7] pig spinal cord. Bands at 46kDa and 48kDa mark correspond to the two major isotypes of CNP protein.