Product Details
Catalog Number: GT22105
Applications: ICC, WB, IHC, IF
Type: Goat IgG
Dilutions: Immunofluorescence: 1:3,000; Immunohistochemistry: 1:3,000; Western Blot: 1:5,000
Immunogen: Full length human recombinant protein expressed in and purified from E. coli
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (polar packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat, Bovine, Porcine
Entrez: 1267
UniProt: P09543
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$299.00Add to Cart

The 2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNP), is an enzyme which catalyzes the hydrolysis of 2′,3′-cyclic nucleotides to 2′-nucleotides. These cyclic nucleotides are structurally different from the better known and studied 3′5′-cyclic nucleotides of which the best known example is cyclic AMP. CNP has two isoforms, CNPase 1 (~46kDa) and CNPase 2 (~48kDa), which are encoded separately by different promoters of the same gene. These enzymes are present in very high levels in brain and peripheral nerve, makes up 4% of total CNS myelin protein. They are found almost exclusively in oligodendrocytes and Schwann cells, appearing early in oligodendrocyte development, earlier than most other myelin specific proteins. Antibodies to CNP have been very useful as a marker for these particular cell types. CNP is thought to play a critical role in the events leading up to myelination, for the oligodendrocytes overexpressing CNP appear to mature earlier in development, resulting in earlier maximum gene expression for myelin basic proteins. It has been reported that CNP is also associated with microtubules in brain tissue and may promote microtubule assembly. CNP can link tubulin to cellular membranes, and may regulate cytoplasmic microtubule distribution.

The antibody was made against the full length recombinant form of human CNP expressed in and purified from E. Coli, and the antibody can be used to identify myelinating cells in cell culture and in sections and to trace axonal projections in sectioned material.

Images
Immunofluorescence

Immunofluorescent analysis of a section of mouse striatum stained with goat pAb to CNP, GT22105, dilution 1:3,000 in green, and costained with rabbit pAb to NF-L in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, the brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies.

Immunofluorescence

Immunofluorescent analysis of a section of mouse striatum stained with goat pAb to CNP, GT22105, dilution 1:3,000 in green, and costained with rabbit pAb to NF-L in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, the brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies.

Western Blot

Western blot analysis of different tissue lysates using goat pAb to CNP, GT22105, dilution 1:5,000, in green: [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] cow spinal cord, and [7] pig spinal cord. Bands at 46kDa and 48kDa mark correspond to the two major isotypes of CNP protein.

Western Blot

Western blot analysis of different tissue lysates using goat pAb to CNP, GT22105, dilution 1:5,000, in green: [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] cow spinal cord, and [7] pig spinal cord. Bands at 46kDa and 48kDa mark correspond to the two major isotypes of CNP protein.