Images
Immunofluorescence

Immunofluorescent analysis of HEK293 cells transfected with mCherry-HA construct (red) and stained with goat pAb to mCherry, GT22108, dilution 1:2,000, in green. The blue is Hoechst staining of nuclear DNA. The GPCA-mCherry antibody reveals mCherry protein expressed only in transfected cells which appear golden in color. Untransfected cells expressing no mCherry are not recognized by the antibody, as a result only their nuclei are visible.

Immunofluorescence

Immunofluorescent analysis of HEK293 cells transfected with mCherry-HA construct (red) and stained with goat pAb to mCherry, GT22108, dilution 1:2,000, in green. The blue is Hoechst staining of nuclear DNA. The GPCA-mCherry antibody reveals mCherry protein expressed only in transfected cells which appear golden in color. Untransfected cells expressing no mCherry are not recognized by the antibody, as a result only their nuclei are visible.

Western Blot

Western blot analysis of HEK293 cell lysates using goat pAb to mCherry, GT22108, dilution 1:1,000, in green, and rabbit pAb to GAPDH, RPCA-GAPDH, dilution 1:5,000, in red: [1] protein standard, [2] untransfected HEK293 control cells, [3] HEK293 cells transfected with pCI-Neo-mod vector expressing two tdTomato protein domains, [4] HEK293 cells transfected with pCI-Neo-mod vector expressing one mCherry-HA protein domain, and [5] HEK293 cells transfected with pCI-Neo-mod vector expressing one GFP domain.

Western Blot

Western blot analysis of HEK293 cell lysates using goat pAb to mCherry, GT22108, dilution 1:1,000, in green, and rabbit pAb to GAPDH, RPCA-GAPDH, dilution 1:5,000, in red: [1] protein standard, [2] untransfected HEK293 control cells, [3] HEK293 cells transfected with pCI-Neo-mod vector expressing two tdTomato protein domains, [4] HEK293 cells transfected with pCI-Neo-mod vector expressing one mCherry-HA protein domain, and [5] HEK293 cells transfected with pCI-Neo-mod vector expressing one GFP domain.

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