The mCherry protein is engineered from a fluorescent protein originally isolated from a coral and is widely used as a tracer in transfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from disc corals of the genus Discosoma.
This antibody was made against full length recombinant mCherry protein expressed in and purified from E. coli. The antibody recognizes mCherry and tdTomato on western blots, in appropriate cells and sections, and does not react with GFP. This antibody can be used to verify the size of fusion constructs by western blotting, and to amplify the endogenous fluorescence of mCherry in transfected cells.
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