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Images
Immunofluorescence analysis of mouse cerebellum section stained with goat pAb to NF-H, GT22109, dilution 1:3,000 in red, and costained with mouse mAb to myelin basic protein (MBP) in green. The NF-H antibody labels axons of basket and Purkinje cells and others. The MBP antibody stains oligodendrocyte cell bodies and the myelin sheathes around axons in the granular layer at center and the white matter at bottom left.
Immunofluorescence analysis of mouse cerebellum section stained with goat pAb to NF-H, GT22109, dilution 1:3,000 in red, and costained with mouse mAb to myelin basic protein (MBP) in green. The NF-H antibody labels axons of basket and Purkinje cells and others. The MBP antibody stains oligodendrocyte cell bodies and the myelin sheathes around axons in the granular layer at center and the white matter at bottom left.
Western blot analysis of tissue lysates from different species using goat pAb to NF-H, GT22109, dilution 1:20,000 in green: [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] cow cerebellum, [5] cow spinal cord, [6] pig hippocampus and [7] pig spinal cord. Strong band at about 220kDa corresponds to the major phospho-NF-H subunit. Smaller proteolytic fragments of NF-H are also detected in some preparations.
Western blot analysis of tissue lysates from different species using goat pAb to NF-H, GT22109, dilution 1:20,000 in green: [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] cow cerebellum, [5] cow spinal cord, [6] pig hippocampus and [7] pig spinal cord. Strong band at about 220kDa corresponds to the major phospho-NF-H subunit. Smaller proteolytic fragments of NF-H are also detected in some preparations.