Product Details
Catalog Number: GT22109
Applications: ICC, WB, IHC, IF
Type: Chicken IgY
Dilutions: Immunofluorescence: 1:1,000-5,000; Immunohistochemistry: 1:1,000-5,000; Western Blot: 1:10,000-25,000
Immunogen: Native NF-H purified from bovine spinal cord
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (polar packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat, Bovine, Porcine
Entrez: 4744
UniProt: P12036
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$299.00Add to Cart

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H, though other proteins may also be present. NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying axonal processes in tissue sections and in culture. NF-H antibodies can also be useful in visualizing neurofilament accumulations seen in many neurological disorders, such as Amyotrophic Lateral Sclerosis (also known as Lou Gehrig’s disease), Alzheimer’s disease and following traumatic injury. The phosphorylated axonal form of NF-H usually referred to as pNF-H, can be detected in blood and CSF following a variety of damage and disease states resulting in axonal compromise, and antibodies such as this can be used to used to quantify such ongoing axonal loss.

This antibody was raised against biochemically isolated NF-H purified from bovine spinal cord. This preparation is dominated by axonal forms of NF-H which are heavily phosphorylated on the multiply repeated NF-H KSP type sequences, and this antibody reacts very strongly with these phosphorylated repeats. Reactivity with non-phosphorylated KSP sequences is orders of magnitude weaker, similar to other characterized antibodies to NF-H. In most species there is some cross-reactivity with the phosphorylated KSP sequences found in the related neurofilament subunit NF-M which are similar but not identical to those of NF-H. The antibody recognizes phosphorylated NF-H strongly in all mammals tested to date and also in chicken.

Images
Immunofluorescence

Immunofluorescence analysis of mouse cerebellum section stained with goat pAb to NF-H, GT22109, dilution 1:3,000 in red, and costained with mouse mAb to myelin basic protein (MBP) in green. The NF-H antibody labels axons of basket and Purkinje cells and others. The MBP antibody stains oligodendrocyte cell bodies and the myelin sheathes around axons in the granular layer at center and the white matter at bottom left.

Immunofluorescence

Immunofluorescence analysis of mouse cerebellum section stained with goat pAb to NF-H, GT22109, dilution 1:3,000 in red, and costained with mouse mAb to myelin basic protein (MBP) in green. The NF-H antibody labels axons of basket and Purkinje cells and others. The MBP antibody stains oligodendrocyte cell bodies and the myelin sheathes around axons in the granular layer at center and the white matter at bottom left.

Western Blot

Western blot analysis of tissue lysates from different species using goat pAb to NF-H, GT22109, dilution 1:20,000 in green: [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] cow cerebellum, [5] cow spinal cord, [6] pig hippocampus and [7] pig spinal cord. Strong band at about 220kDa corresponds to the major phospho-NF-H subunit. Smaller proteolytic fragments of NF-H are also detected in some preparations.

Western Blot

Western blot analysis of tissue lysates from different species using goat pAb to NF-H, GT22109, dilution 1:20,000 in green: [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] cow cerebellum, [5] cow spinal cord, [6] pig hippocampus and [7] pig spinal cord. Strong band at about 220kDa corresponds to the major phospho-NF-H subunit. Smaller proteolytic fragments of NF-H are also detected in some preparations.