Product Details
Catalog Number: HEC01
Applications: Cell Assays
Type: Primary Cells
Species Reactivity: Human
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
500,000+ Cells frozen$299.00Add to Cart
Culturing Protocol - T25 Flask

HUVECs were isolated from normal human umbilical vein. Passage 1, cells are shipped frozen or in proliferating culture with a confluency of >90 %. ENDO-Growth medium containing 5% serum and growth supplements are recommended for culture. Cells have an average additional population doubling levels >20 when cultured.

Image: HUVECs cultured using our Collagel Hydrogel.

Characterization of the cells
  • Cytoplasmic VWF / Factor VIII: >95% positive by immunofluorescence
  • Cytoplasmic uptake of Di-I-Ac-LDL: >95% positive by immunofluorescence
  • Cytoplasmic PECAM1 >95% positive by immunofluorescence

HUVECs are negative for HIV-1, HBV, HCV, and mycoplasma.

Supporting Products
NameCatalog #
AlphaBioCoat SolutionAC001
Cell Detachment SolutionADF001
ENDO-Growth Media KitEGK001
ENDO-Growth SupplementsEGS001
Endothelial Cryopreserve MediaFM300
ENDO-Growth MediaMED001
ENDO Basal MediaMED002
1X Phosphate Buffer SolutionPBS300
Smooth Coat SolutionSC300
Images