HBMVECs cells were isolated from the normal human cerebral cortex. HBMVEC-GFP-AEQ were selected by puromycin from HBMVECs after infected with lentiviruses expressing GFP-AEQOURIN in mitochondria. The cells are shipped in frozen vials (the cells are provided @ passage 1). Endo-Growth Media (MED001, contains 10% serum and growth supplements) is recommended for cell culture and these cells have a minimum average population doubling levels > 15 when cultured following the detailed protocol described below).
Cells are prepared and manufactured in an ISO 9001:2015 certified environment and ISO 5 cleanroom.
|ENDO Basal Media||MED002||500 ml|
|ENDO-Growth Media||MED001||500 ml|
|Smooth Coat Solution||SC300||20 ml|
|Universal Detachment Solution||AD002||100 ml|
|Universal Neutralization Buffer||NB001||100 ml|
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