HBMVECs cells were isolated from the normal human cerebral cortex. HBMVEC-VE-CAD-GFP were selected by puromycin from HBMVECs after infected with lentiviruses expressing VE-CAD-GFP the cells are shipped in frozen vials (the cells are provided @ passage 1). Endo-Growth Media (MED001, contains 10% serum and growth supplements) is recommended for cell culture and these cells have a minimum average population doubling levels > 15 when cultured following the detailed protocol described below).
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