Product Details
Catalog Number: HEC08
Applications: Cell Assays
Type: Primary Cells
Storage: Keep frozen in liquid nitrogen until plating
Shipping: Frozen (Dry Ice/Liquid Nitrogen)
Species Reactivity: Human
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
500,000 + Cells Cryopreserved$789.00Add to Cart

HPMECs were initiated by elutriation from dissociated normal human pancreatic tissue. Passage 3 cells are shipped frozen. ENDO-Growth medium (MED001) is recommended for culture. Cells have an average additional population doubling levels >20 when cultured.

Characterization of the cells
  • Cytoplasmic VWF / Factor VIII: >95% positive by immunofluorescence
  • Cytoplasmic uptake of Di-I-Ac-LDL: >95% positive by immunofluorescence
  • Cytoplasmic PECAM1 >95% positive by immunofluorescence

HPMECs are negative for HIV-1, HBV, HCV, and mycoplasma.

Images
HEC08

Human Pancreatic Microvascular Endothelial Cells (HEC08) are stained with vWF (red) and CD31 (green). Nuclei counterstained blue with DAPI. As you can see, CD31 is on cell membrane whereas vWF is in the cytoplasm.

HEC08

Human Pancreatic Microvascular Endothelial Cells (HEC08) are stained with vWF (red) and CD31 (green). Nuclei counterstained blue with DAPI. As you can see, CD31 is on cell membrane whereas vWF is in the cytoplasm.