Product Details
Catalog Number: HEC16
Applications: Cell Assays
Type: Primary Cells
Storage: Keep frozen in liquid nitrogen until plating
Shipping: Frozen (Dry Ice/Liquid Nitrogen)
Species Reactivity: Human
Downloads: Datasheet (pdf)
Product Sizes
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500,000+ Cells Frozen$789.00Add to Cart

HLIMECs were initiated by elutriation from dissociated normal large intestine tissue. Passage 3 cells are shipped cryopreserved. ENDO-Growth Medium (MED001) is recommended for culture. Cells have an average additional population doubling levels >16 when cultured. 

Characterization of the cells
  • Cytoplasmic VWF / Factor VIII: >95% positive by immunofluorescence
  • Cytoplasmic uptake of Di-I-Ac-LDL: >95% positive by immunofluorescence
  • Cytoplasmic PECAM1 >95% positive by immunofluorescence

HLIMECs are negative for HIV-1, HBV, HCV, and mycoplasma.

Images
HEC16

Staining of cells with CD31 in red and vWF in green. Cells were counterstained with DAPI (cell nuclei). CD31 is distributed on cell membrane whereas vWF is in cytoplasm as expected.

HEC16

Staining of cells with CD31 in red and vWF in green. Cells were counterstained with DAPI (cell nuclei). CD31 is distributed on cell membrane whereas vWF is in cytoplasm as expected.

Human Large Intestine Microvascular Endothelial Cells in two days in culture.

Human Large Intestine Microvascular Endothelial Cells in two days in culture.

Human Large Intestine Microvascular Endothelial Cells in two days in culture.

Human Large Intestine Microvascular Endothelial Cells in two days in culture.