HLIMECs were initiated by elutriation from dissociated normal large intestine tissue. Passage 3 cells are shipped cryopreserved. ENDO-Growth Medium (MED001) is recommended for culture. Cells have an average additional population doubling levels >16 when cultured.
Characterization of the cells
HLIMECs are negative for HIV-1, HBV, HCV, and mycoplasma.
|1X Phosphate Buffer Solution||PBS300||100 ml|
|AlphaBioCoat Solution||AC001||20 ml|
|Cell Detachment Solution||ADF001||50 ml|
|ENDO Basal Media||MED002||500 ml|
|ENDO-Growth Media||MED001||500 ml|
|ENDO-Growth Media Kit||EGK001||500 ml|
|ENDO-Growth Supplements||EGS001||25 ml|
|Endothelial Cryopreserve Media||FM300||10 ml|
|Smooth Coat Solution||SC300||20 ml|
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