Product Details
Catalog Number: HEC16
Applications: Cell Assays
Type: Primary Cells
Species Reactivity: Human
Downloads: Datasheet (pdf)
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HLIMECs were initiated by elutriation from dissociated normal large intestine tissue. Passage 3 cells are shipped cryopreserved. ENDO-Growth Medium (MED001) is recommended for culture. Cells have an average additional population doubling levels >16 when cultured.

Image: Human Large Intestine Microvascular Endothelial Cells in two days in culture. 

Characterization of the cells
  • Cytoplasmic VWF / Factor VIII: >95% positive by immunofluorescence
  • Cytoplasmic uptake of Di-I-Ac-LDL: >95% positive by immunofluorescence
  • Cytoplasmic PECAM1 >95% positive by immunofluorescence

HLIMECs are negative for HIV-1, HBV, HCV, and mycoplasma.

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