Product Details
Catalog Number: MO22101
Applications: ICC, WB, IHC, IF
Type: Mouse IgG
Dilutions: Immunofluorescent: 1:1,000 Immunocytochemistry: 1:1,000 Immunohistochemistry: 1:1,000 Western Blot: 1:10,000
Immunogen: GFAP isolated biochemically from pig spinal cord
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat, Bovine, Porcine
Entrez: 2670
UniProt: P14136
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$303.00Add to Cart

Glial Fibrillary Acidic Protein (GFAP) is strongly and specifically expressed in astrocytes, Bergmann glia and certain other glia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves (1-3). GFAP expression is also seen in developing neural stem cells and GFAP levels may greatly increase in regions of CNS injury or disease (4), and point mutations in the GFAP gene are causative of Alexander's disease (5). Antibodies to GFAP such as MCA-2A5 are useful for visualizing glia and monitoring developmental, disease and damage related CNS alterations.

Images
Immunofluorescent

Immunofluorescent analysis of an adult rat cerebellum section stained with mouse mAb to GFAP, MO22101, dilution 1:500, in red, and costained with chicken pAb to parvalbumin, dilution 1:2,000, in green. The blue is DAPI staining of nuclear DNA.

Immunofluorescent

Immunofluorescent analysis of an adult rat cerebellum section stained with mouse mAb to GFAP, MO22101, dilution 1:500, in red, and costained with chicken pAb to parvalbumin, dilution 1:2,000, in green. The blue is DAPI staining of nuclear DNA.

Western Blot

Western blot analysis of equal amount of total protein from different tissue lysates and recombinant proteins solutions using mouse mAb to GFAP dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig brain, [7] rat recombinant GFAP, [8] human recombinant GFAP. Bands around 50kDa correspond to alternative transcripts and proteolytic products of GFAP.

Western Blot

Western blot analysis of equal amount of total protein from different tissue lysates and recombinant proteins solutions using mouse mAb to GFAP dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig brain, [7] rat recombinant GFAP, [8] human recombinant GFAP. Bands around 50kDa correspond to alternative transcripts and proteolytic products of GFAP.