Product Details
Catalog Number: MO22105
Applications: ICC, WB, IHC
Type: Mouse IgG
Dilutions: ICC 1:100; IHC 1:100; WB 1:1,000
Immunogen: C-terminus of rat NF-M recombinant protein
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Supernatant
Format B: liquid
Species Reactivity: Human, Mouse, Rat, Chicken
Entrez: 4741
UniProt: P07197
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$248.00Add to Cart

Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. In the electron microscope neurofilaments appears as 10nm diameter fibres of indeterminate length which generally have fine wispy protrusions from their sides. They are found particularly abundantly in axons of large projection neurons. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable.

Images

Culture of adult neural cells. Mature neurons can be identified by their morphology and because they stain strongly with antibodies to NF-L, NF-M and NF-H. The surrounding stellate red cells are stained with Neurofilament alpha-internexin/NF66. These are apparently mitotic neuronal progenitor cells and express many other neuronal markers.

Culture of adult neural cells. Mature neurons can be identified by their morphology and because they stain strongly with antibodies to NF-L, NF-M and NF-H. The surrounding stellate red cells are stained with Neurofilament alpha-internexin/NF66. These are apparently mitotic neuronal progenitor cells and express many other neuronal markers.

Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with various antibodies including NF-M (lane 3).

Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with various antibodies including NF-M (lane 3).

Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with various antibodies including NF-M (lane 3).

Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with various antibodies including NF-M (lane 3).