Product Details
Catalog Number: MO22110
Applications: WB, IF
Type: Mouse IgG
Dilutions: ICC 1:1000; IHC 1:1000; WB 1:10,000
Immunogen: Recombinant protein construct containing the 7th, 8th and 9th spectrin repeats of human alpha-II spectrin (amino acids 676-1043. This construct was expressed in and purified from E. coli.
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Mouse, Rat
Entrez: 6709
UniProt: Q13813
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$325.00Add to Cart

The spectrin family of proteins were originally discovered as major components of the submembraneous cytoskeleton of osmotically lysed red blood cells. Work by Levine and Willard described a pair of about ~240-260kDa molecular weight bands which were transported at the slowest rate along mammalian axons. They named these proteins "fodrin" as antibody studies showed that they were localized in the sheath under the axonal membrane, but not in the core of the axon.

In the nervous system, alpha-II-spectrin or alpha-Fodrin is found predominantly in neurons. Our antibody can therefore be used to identify neurons and fragments derived from neuronal membranes in cells in tissue culture and in sectioned material. This antibody was raised against a recombinant construct containing the seventh, eighth and ninth of the so-called spectrin repeats.

Images

Mixed neuron-glial cultures stained with alpha-II spectrin (green) and counterstained for DNA (blue). Alpha-II spectrin stains numerous axonal and dendritic profiles. This image shows an optical section through a group of neuronal cell bodies and reveals the submembraneous cytoskeleton. Since alpha-II spectrin is specific for neurons in the CNS, the glial cells in this culture are not recognized. This antibody also reveals the submembranous cytoskeleton of the axon. Protocol on data-sheet.

Mixed neuron-glial cultures stained with alpha-II spectrin (green) and counterstained for DNA (blue). Alpha-II spectrin stains numerous axonal and dendritic profiles. This image shows an optical section through a group of neuronal cell bodies and reveals the submembraneous cytoskeleton. Since alpha-II spectrin is specific for neurons in the CNS, the glial cells in this culture are not recognized. This antibody also reveals the submembranous cytoskeleton of the axon. Protocol on data-sheet.

Western blots of extract of bovine spinal cord. A prominent band running at 240kDa respresents the intact alpha-II spectrin heavy chain. Protocol on data-sheet..

Western blots of extract of bovine spinal cord. A prominent band running at 240kDa respresents the intact alpha-II spectrin heavy chain. Protocol on data-sheet..