Product Details
Catalog Number: MO22113
Applications: WB, IF
Type: Mouse IgG
Dilutions: ICC 1:1000; IHC 1:1000; WB 1:10,000
Immunogen: Recombinant full length human Doublecortin purified from E. coli.
Storage: Antibody can also be aliquotted and stored frozen at -20° C to -70° C in a manual defrost freezer for six months without detectable loss of activity. The antibody can be stored at 2° - 8° C for 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles
Format A: Supernatant
Format B: liquid
Species Reactivity: Human, Mouse, Rat, Bovine, Porcine
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$295.00Add to Cart

Doublecortin (DCX) has a molecular weight of about 45kDa. The protein contains two domains, each about 90 amino acids long, which are believed to function in binding to microtubules, a C-terminal serine and proline rich region which may become phosphorylated in vivo. DCX is expressed very early in neuronal development, as neuroblasts become post-mitotic, but is lost as neurons mature. Developing neurons start to lose DCX expression about the time that they begin to express NeuN, a neuronal specific RNA binding protein characteristic of more mature neurons. Antibodies to DCX are used to identify stem cells in sections and in tissue culture, and to see if neurogenesis is taking place.

Defects in DCX are the cause of lissencephaly X-linked type 1 (LISX1) [MIM:300067]; also called X-LIS or LIS. LISX1 is a classic lissencephaly characterized by mental retardation and seizures that are more severe in male patients.

Images

E18 hippocampal neurons stained with MAPT (red) and Doublecortin (green). The two proteins overlap in the proximal dendrites, but doublecortin is more abundant in the growth cones and periphery. As a result, the periphery appears green while the more proximal regions of the cells are yellow. The single longer process of this cell, presumably an axon, has a low doublecortin content and so appears red. Blue staining is the nuclear DNA. Protocol on datasheet.

E18 hippocampal neurons stained with MAPT (red) and Doublecortin (green). The two proteins overlap in the proximal dendrites, but doublecortin is more abundant in the growth cones and periphery. As a result, the periphery appears green while the more proximal regions of the cells are yellow. The single longer process of this cell, presumably an axon, has a low doublecortin content and so appears red. Blue staining is the nuclear DNA. Protocol on datasheet.

Blots of crude rat brain extract stained with Doublecortin. Protocol on datasheet.

Blots of crude rat brain extract stained with Doublecortin. Protocol on datasheet.