Product Details
Catalog Number: MO22115
Applications: ICC, WB, IHC, IF
Type: Mouse IgG
Dilutions: Immunofluorescent: 1:1,000 Immunocytochemistry: 1:1,000 Immunohistochemistry: 1:1,000 Western Blot: 1:10,000
Immunogen: Full length recombinant human vimentin protein expressed in and purified from E. coli.
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: liquid
Species Reactivity: Human, Rat
Entrez: 7431
UniProt: P08670
Downloads: Datasheet (pdf)
Product Sizes
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100 ul of 1 mg/ml$275.00Add to Cart

Vimentin is a protein which assembles to produce 10mm or intermediate filaments, which are major components of the cellular cytoskeleton. This protein is expressed in microglia, developing astrocytes, developing neurons, fibroblasts and endothelial cells in the developing nervous system. Levels of vimentin generally are reduced as development proceeds and in adult animals vimentin is mostly found in mesenchymal tissues. Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. The immunogen used to generate our antibody was recombinant human vimentin expressed in and purified from E. coli.

Images
Immunofluorescent

Immunofluorescent analysis of cortical neuron-glial cell cultures from E20 rat stained with mouse mAb to vimentin dilution 1:2,000 in red, and costained with chicken pAb to glial fibrillary acidic protein (GFAP) dilution 1:5,000, in green. The blue is DAPI staining of nuclear DNA. Fibroblastic and other developing cells express only vimentin and appear red. Astrocytes that express GFAP only are green while those that express both GFAP and vimentin appear golden yellow.

Immunofluorescent

Immunofluorescent analysis of cortical neuron-glial cell cultures from E20 rat stained with mouse mAb to vimentin dilution 1:2,000 in red, and costained with chicken pAb to glial fibrillary acidic protein (GFAP) dilution 1:5,000, in green. The blue is DAPI staining of nuclear DNA. Fibroblastic and other developing cells express only vimentin and appear red. Astrocytes that express GFAP only are green while those that express both GFAP and vimentin appear golden yellow.

Western Blot

Western blot analysis of different cell lysates using mouse mAb to vimentin dilution 1:10,000 in green: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS-1, and [6] C6 cells. The band at about 50kDa mark corresponds to the vimentin protein.

Western Blot

Western blot analysis of different cell lysates using mouse mAb to vimentin dilution 1:10,000 in green: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS-1, and [6] C6 cells. The band at about 50kDa mark corresponds to the vimentin protein.