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- Catherine Reed-McBain, Rithvik V. Turaga, Seth R.T. Zima, Janmesh Patel, Anderson Weber Faletti Cunha, Jason Mixdorf, Lauren E Wehner, Jonathan W. Engle, Reinier Hernandez, Stevens K Rehen, Helena L Borges, and Jose M Ayuso. (2025). Non-Destructive Luminescence and PET Imaging to Monitor Tissue Microenvironment in Microphysiological Systems During Brain Metastasis Using Dissociated Cerebral Organoids. Biofabrication. doi: 10.1088/1758-5090/ade1fb
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Images
Mixed neuron/glia cultures stained with MAP-2 (green) and also rabbit antibody of neurofilament NF-H (red). Since the NF-H protein is largely expressed in neuronal axons, while the MAP2 is only found in neuronal dendrites and perikarya, there is little overlap between these two staining patterns. DNA stain shows nuclei of neurons and non-neuronal cells (blue).Protocol on data-sheet.
Mixed neuron/glia cultures stained with MAP-2 (green) and also rabbit antibody of neurofilament NF-H (red). Since the NF-H protein is largely expressed in neuronal axons, while the MAP2 is only found in neuronal dendrites and perikarya, there is little overlap between these two staining patterns. DNA stain shows nuclei of neurons and non-neuronal cells (blue).Protocol on data-sheet.
Western blot of whole rat brain lysate probed with antibody to MAP2. Note that the strong single band running at about 280Kda corresponds to MAP2. Protocol on data-sheet.
Western blot of whole rat brain lysate probed with antibody to MAP2. Note that the strong single band running at about 280Kda corresponds to MAP2. Protocol on data-sheet.