Product Details
Catalog Number: MO22116
Applications: WB, IF
Type: Mouse IgG
Dilutions: ICC 1:1,000; IHC 1:1,000; WB 1:10,000
Immunogen: High molecular MAP protein preparation derived from bovine brain.
Storage: Antibody can also be aliquotted and stored frozen at -20° C to -70° C in a manual defrost freezer for six months without detectable loss of activity. The antibody can be stored at 2° - 8° C for 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Mouse, Rat, Bovine
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$295.00Add to Cart

Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein tau and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein tau.

MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites. In contrast tau is found predominantly in neuronal axons.

Images

Mixed neuron/glia cultures stained with MAP-2 (green) and also rabbit antibody of neurofilament NF-H (red). Since the NF-H protein is largely expressed in neuronal axons, while the MAP2 is only found in neuronal dendrites and perikarya, there is little overlap between these two staining patterns. DNA stain shows nuclei of neurons and non-neuronal cells (blue).Protocol on data-sheet.

Mixed neuron/glia cultures stained with MAP-2 (green) and also rabbit antibody of neurofilament NF-H (red). Since the NF-H protein is largely expressed in neuronal axons, while the MAP2 is only found in neuronal dendrites and perikarya, there is little overlap between these two staining patterns. DNA stain shows nuclei of neurons and non-neuronal cells (blue).Protocol on data-sheet.

Western blot of whole rat brain lysate probed with antibody to MAP2. Note that the strong single band running at about 280Kda corresponds to MAP2. Protocol on data-sheet.

Western blot of whole rat brain lysate probed with antibody to MAP2. Note that the strong single band running at about 280Kda corresponds to MAP2. Protocol on data-sheet.