Product Details
Catalog Number: MO22121
Applications: WB, IF
Type: Mouse IgG
Dilutions: ICC 1:2000; IHC 1:2000; WB 1:10,000
Immunogen: Mixture of 3 MBP peptides which are the different forms of the protein which are alternate transcription from a single gene.
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Mouse, Rat
Entrez: 4155
UniProt: P02686
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$303.00Add to Cart

Myelin basic protein is reported to account for about 30 of the proteins in myelin found in the central nervous system. It can induce experimental allergic encephalomyelitis (EAE), a T-lymphocyte mediated disease due to delayed-type hypersensitivity; though each animal species appears to respond to a different fragment of the 170 amino acid polypeptide. Four different isoforms have been identified through cDNA cloning. All four of these variants are identical except for the insertion or deletion of two peptide fragments encoded by exons 2 and 5. Myelin basic protein is reported to be expressed in oligodendrocytes, myelin of white matter in the brain and spinal cord and in peripheral nerves, though it is expressed less abundantly in gray matter.

MBP has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. In vitro, MBP is suitable as a substrate for numerous protein kinases, including the ERK and p38 MAP kinases that phosphorylate MBP at T98.

Images
IHC Image (Above right). Mouse striatum. Dilution 1:500. Image courtesy of Katherine Given. University of Colorado

IHC Image (Above right). Mouse striatum. Dilution 1:500. Image courtesy of Katherine Given. University of Colorado

IHC Image (Above right). Mouse striatum. Dilution 1:500. Image courtesy of Katherine Given. University of Colorado

IHC Image (Above right). Mouse striatum. Dilution 1:500. Image courtesy of Katherine Given. University of Colorado

Staining in mouse cortex, corpus callosum, and striatum

Staining in mouse cortex, corpus callosum, and striatum. Image and protocol courtesy of Sean Hickey and Katherine Given, University of Colorado.

Staining in mouse cortex, corpus callosum, and striatum

Staining in mouse cortex, corpus callosum, and striatum. Image and protocol courtesy of Sean Hickey and Katherine Given, University of Colorado.

Shows rat mixed neuron/glial cultures stained with Myelin Basic Protein antibody (red), and also with chicken antibody to neurofilament NF-L (green). Blue is a DNA stain. Note that the MBP antibody stains an oligodendrocyte and some membrane shed from this cell. Other cells in the field include neurons, astrocytes, microglia and fibroblasts, all of which are completely negative for MBP, though the neuronal processes can be seen with the NF-L antibody. Protocol on data-sheet.

Shows rat mixed neuron/glial cultures stained with Myelin Basic Protein antibody (red), and also with chicken antibody to neurofilament NF-L (green). Blue is a DNA stain. Note that the MBP antibody stains an oligodendrocyte and some membrane shed from this cell. Other cells in the field include neurons, astrocytes, microglia and fibroblasts, all of which are completely negative for MBP, though the neuronal processes can be seen with the NF-L antibody. Protocol on data-sheet.

Blots of crude rat spinal cord homogenate blotted with MBP-Mouse Monoclonal Antibody. Protocol on data-sheet.

Blots of crude rat spinal cord homogenate blotted with MBP-Mouse Monoclonal Antibody. Protocol on data-sheet.