Product Details
Catalog Number: MO22125
Applications: WB, IF
Type: Mouse IgG
Immunogen: Actin preparation derived from bovine brain.
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Mouse, Rat, Bovine, Porcine
Entrez: 4000
UniProt: P02545
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
100 ug$275.00Add to Cart

Actin is one of the most abundant and highly conserved proteins of eukaryotes. Mammalian actins are the product of six different genes with differing distribution patterns in cell types and in tissues. The molecular weight of all six proteins is 42kDa, and one or more actins is found in essentially every type of crude cellular and tissue extract. Actin is regarded as a "housekeeping" protein which is generally not altered much in expression as a result of experimental manipulations. So quantitation of the actin band on the western blots is used as a standard against which the band density of other proteins can be compared. Glyceraldehyde 3 phosphate dehydrogenase (GAPDH)(MO25038) is another "housekeeping" protein used in this way. Since our antibody binds all six isotypes very strongly on western blots, it is a very effective blotting standard. It also works in immunocytochemical experiments, binding strongly and cleanly to filopodia, membrane ruffles and stress fibers, all known to be rich in actin

Actin is also a marker for autoimmune diseases of the gut and correlates to intestinal damage. It is also associated with certain forms of hepatitis.

  • Product Reviews

    Leave a review for this product and you could be eligible for a $10 Starbucks gift card. 

  • Product Publications
    Product Publications

    If you've used this product in a publication, let us know. Email rose@neuromics.com, with the publication details and you could be eligible for an Amazon gift card.  

  • Related Products
    Related Products
    NameCatalog #Size
    GAPDHMO25038100 ul
Images

HeLa cells stained with Actin (red) and also with our chicken polyclonal antibody to Vimentin, (green) (CH22108). The actin antibody stains the submembranous actin rich cytoskeleton and also stress fibers, bundles of actin associated with adhesion sites. The vimentin antibody stains a quite different cytoskeletal network, the intermediate filaments. The blue stain reveals DNA in the nuclei of these cells. Protocol on Datasheet.

HeLa cells stained with Actin (red) and also with our chicken polyclonal antibody to Vimentin, (green) (CH22108). The actin antibody stains the submembranous actin rich cytoskeleton and also stress fibers, bundles of actin associated with adhesion sites. The vimentin antibody stains a quite different cytoskeletal network, the intermediate filaments. The blue stain reveals DNA in the nuclei of these cells. Protocol on Datasheet.

Western blot of crude extract of the human carcinoma HeLa cell line. Lane 15 was probed with Actin, our monoclonal antibody to all six actin isotypes, giving a strong clean band at an SDS-PAGE molecular weight of 42kDa. lane 16 was probed with our antibody to 14-3-3η (14-3-3 eta) (MO22126), which has an SDS-PAGE molecular weight of 28kDa. Lanes 17 was probed with our monoclonal antibody to vimentin (CH22108). Protocol on Datasheet.

Western blot of crude extract of the human carcinoma HeLa cell line. Lane 15 was probed with Actin, our monoclonal antibody to all six actin isotypes, giving a strong clean band at an SDS-PAGE molecular weight of 42kDa. lane 16 was probed with our antibody to 14-3-3η (14-3-3 eta) (MO22126), which has an SDS-PAGE molecular weight of 28kDa. Lanes 17 was probed with our monoclonal antibody to vimentin (CH22108). Protocol on Datasheet.