Product Details
Catalog Number: MO22129
Applications: ICC, WB, IHC, IF
Type: Mouse IgG
Dilutions: Immunofluorescent: 1:2,000 Immunocytochemistry: 1:2,000 Immunohistochemistry: 1:2,000 Western Blot: 1:10,000
Immunogen: amino acids 32-350 of the human LAMP1 precursor sequence in NP_005552.3purified from E. coli
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: liquid
Species Reactivity: Human
Entrez: 3916
UniProt: P11279
Downloads: Datasheet (pdf)
Product Sizes
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100 ul of 1 mg/ml$275.00Add to Cart

LAMP1 (lysosomal associated membrane protein 1) is a protein primarily associated with the lysosomal membrane. Antibodies to LAMP1 are therefore excellent markers of lysosomes in mammalian cells, though some LAMP1 may also be seen on late endosomes and on the plasma membrane. The protein is also known as CD107a, lysosomal associated membrane glycoprotein 1, LGP120 and LAMPA, as the protein was independently discovered and named by several different labs. This antibody can be used to visualize lysosomes in human cells and to quantify lysosomal content in human cells by western blotting. Mouse select image at left for larger view.

Images
Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with mouse mAb to LAMP1, MO22129, dilution 1:500 in red, and costained with chicken pAb to vimentin dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA.

Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with mouse mAb to LAMP1, MO22129, dilution 1:500 in red, and costained with chicken pAb to vimentin dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA.

Western Blot

Western blot analysis of different cell lysates using mouse mAb to LAMP1, MO22129, dilution 1:10,000 in green. Cells were maintained under normal conditions (Ct), or treated with 50µM of chloroquine (CQ), an inhibitor of autophagy, for 24 hours: [1] protein standard (red), [2] HeLa Ct, [3] HeLa+CQ, [4] NIH-3T3 Ct, and [5] NIH-3T3+CQ

Western Blot

Western blot analysis of different cell lysates using mouse mAb to LAMP1, MO22129, dilution 1:10,000 in green. Cells were maintained under normal conditions (Ct), or treated with 50µM of chloroquine (CQ), an inhibitor of autophagy, for 24 hours: [1] protein standard (red), [2] HeLa Ct, [3] HeLa+CQ, [4] NIH-3T3 Ct, and [5] NIH-3T3+CQ