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Immunofluorescent analysis of HeLa cells stained with mouse mAb to LAMP1, MO22129, dilution 1:500 in red, and costained with chicken pAb to vimentin dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA.
Immunofluorescent analysis of HeLa cells stained with mouse mAb to LAMP1, MO22129, dilution 1:500 in red, and costained with chicken pAb to vimentin dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA.
Western blot analysis of different cell lysates using mouse mAb to LAMP1, MO22129, dilution 1:10,000 in green. Cells were maintained under normal conditions (Ct), or treated with 50µM of chloroquine (CQ), an inhibitor of autophagy, for 24 hours: [1] protein standard (red), [2] HeLa Ct, [3] HeLa+CQ, [4] NIH-3T3 Ct, and [5] NIH-3T3+CQ
Western blot analysis of different cell lysates using mouse mAb to LAMP1, MO22129, dilution 1:10,000 in green. Cells were maintained under normal conditions (Ct), or treated with 50µM of chloroquine (CQ), an inhibitor of autophagy, for 24 hours: [1] protein standard (red), [2] HeLa Ct, [3] HeLa+CQ, [4] NIH-3T3 Ct, and [5] NIH-3T3+CQ