Product Details
Catalog Number: MO22134
Applications: WB, IHC, IF
Type: Mouse IgG
Immunogen: Full length recombinant human HMGB1 protein
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Mouse, Porcine, Multiple Mammalian
Entrez: 3146
UniProt: P09429
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$325.00Add to Cart

High-mobility group protein B1 or HMGB1 is among the most important chromatin proteins. In the nucleus, HMGB1 interacts with nucleosomes, transcription factors, and histones.[3] This nuclear protein organizes the DNA and regulates transcription.

It is released by activated macrophages, and serum levels increase significantly during endotoxaemia, sepsis, and arthritis with significant delayed kinetics in comparison with tumour necrosis factor (TNF) and interleukin-1beta. Recently identified biological activities of HMGB1 include activation of macrophages/monocytes to release proinflammatory cytokines, upregulation of endothelial adhesion molecules, stimulation of epithelial cell barrier failure, and mediation of fever and anorexia. Passive immunization with anti-HMGB1 antibodies confers significant protection against lethal endotoxaemia, sepsis, arthritis and lipopolysaccharide-induced acute lung injury.

Images

HeLa cells grown in tissue culture and stained with HMGB1 (red), Neuromics’ chicken polyclonal antibody to Vimentin (Cat#CH22108) (green) and DNA (blue). The HMGB1 antibody reveals strong nuclear staining which overlaps with the DNA stain. Protocol on Datasheet.

HeLa cells grown in tissue culture and stained with HMGB1 (red), Neuromics’ chicken polyclonal antibody to Vimentin (Cat#CH22108) (green) and DNA (blue). The HMGB1 antibody reveals strong nuclear staining which overlaps with the DNA stain. Protocol on Datasheet.

Blot of crude HeLa cell extract. HMGB1 runs at an apparent molecular weight of 25kDa. Protocol on Datasheet.

Blot of crude HeLa cell extract. HMGB1 runs at an apparent molecular weight of 25kDa. Protocol on Datasheet.