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| Size | List Price | Price | Cart |
|---|---|---|---|
| 100 ul | $295.00 | Add to Cart |
Aldolase A is generally considered to be a muscle enzyme. Northern analysis of cultured cells suggests that it is present in both neurons and glia. Aldolase C shares 81% amino acid identity with aldolase A and 70% identity with aldolase B. Earlier studies using isozyme-specific antibodies report its location in gray matter astrocytes and cells of the pia mater. In situ hybridization of mouse central nervous system using isozyme-specific probes revealed that aldolase A and C are expressed in complementary cell types: aldolase A mRNA is found in neurons; aldolase C message is detected in astrocytes, some cells of the pia mater, and Purkinje cells. Aldolase C can in some situations be used as an astrocyte marker. However Purkinje cells of the cerebellum contain high levels of the enzyme, so the enzyme is not totally astrocyte specific. |
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Images
Left: View of mixed neuron/glial cultures stained with Aldolase-C (green) and our rabbit antibody to NeuN/FOX3 (red). Middle and Right: Mouse brain sections (fixed by transcardial perfusion with 4% paraformaldehyde) stained with Aldolase-C (red) and our chicken Vimentin antibody (green). Nuclei are labeled with DAPI (blue).
Left: View of mixed neuron/glial cultures stained with Aldolase-C (green) and our rabbit antibody to NeuN/FOX3 (red). Middle and Right: Mouse brain sections (fixed by transcardial perfusion with 4% paraformaldehyde) stained with Aldolase-C (red) and our chicken Vimentin antibody (green). Nuclei are labeled with DAPI (blue).
Blots of crude cow cerebellum homogenate blotted with Aldolase-C. The monoclonal antibody binds strongly and cleanly to a band at about 40 kDa. Other studies show that this antibody does not recognize the very closely related molecules aldolase A or B.
Blots of crude cow cerebellum homogenate blotted with Aldolase-C. The monoclonal antibody binds strongly and cleanly to a band at about 40 kDa. Other studies show that this antibody does not recognize the very closely related molecules aldolase A or B.