Product Details
Catalog Number: MO22135
Applications: WB, IF
Type: Mouse IgG
Immunogen: MPHSYPALSAEQKKELSDIA
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Protein G Purified
Format B: liquid
Species Reactivity: Human, Mouse, Rat, Bovine, Porcine, Multiple Mammalian
Entrez: 230
UniProt: P09972
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$325.00Add to Cart

Aldolase A is generally considered to be a muscle enzyme. Northern analysis of cultured cells suggests that it is present in both neurons and glia. Aldolase C shares 81% amino acid identity with aldolase A and 70% identity with aldolase B.

Earlier studies using isozyme-specific antibodies report its location in gray matter astrocytes and cells of the pia mater. In situ hybridization of mouse central nervous system using isozyme-specific probes revealed that aldolase A and C are expressed in complementary cell types: aldolase A mRNA is found in neurons; aldolase C message is detected in astrocytes, some cells of the pia mater, and Purkinje cells. Aldolase C can in some situations be used as an astrocyte marker. However Purkinje cells of the cerebellum contain high levels of the enzyme, so the enzyme is not totally astrocyte specific.

Images

Left: View of mixed neuron/glial cultures stained with Aldolase-C (green) and our rabbit antibody to NeuN/FOX3 (red). Middle and Right: Mouse brain sections (fixed by transcardial perfusion with 4% paraformaldehyde) stained with Aldolase-C (red) and our chicken Vimentin antibody (green). Nuclei are labeled with DAPI (blue).

Left: View of mixed neuron/glial cultures stained with Aldolase-C (green) and our rabbit antibody to NeuN/FOX3 (red). Middle and Right: Mouse brain sections (fixed by transcardial perfusion with 4% paraformaldehyde) stained with Aldolase-C (red) and our chicken Vimentin antibody (green). Nuclei are labeled with DAPI (blue).

Blots of crude cow cerebellum homogenate blotted with Aldolase-C. The monoclonal antibody binds strongly and cleanly to a band at about 40 kDa. Other studies show that this antibody does not recognize the very closely related molecules aldolase A or B.

Blots of crude cow cerebellum homogenate blotted with Aldolase-C. The monoclonal antibody binds strongly and cleanly to a band at about 40 kDa. Other studies show that this antibody does not recognize the very closely related molecules aldolase A or B.