Product Details
Catalog Number: MO22136
Applications: WB, IHC, IF
Type: Mouse IgG
Immunogen: Recombinant GFAP and purified bovine GFAP
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Mouse, Rat, Primate, Chicken, Rabbit, Bovine, Cat, Dog, Porcine, Equine
Entrez: 2670
UniProt: P14136
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$325.00Add to Cart

Human GFAP is a 49,749 dalton protein (432 amino acids) expressed in astrocytes. GFAP is an intermediate filament protein and acts as an intracellular structural component of the cytoskeleton. During embryonic and fetal life, GFAP is also expressed by radial glial cells of the CNS. Various mutations of the GFAP gene in humans produce Alexander's disease, one of the leukodystrophies.

Antibodies to GFAP are therefore very useful as markers of astrocytic cells and neural stem cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP.

Images

Mixed neuron-glial cultures stained with Mouse Monoclonal GFAP, and Chicken Polyclonal Neurofilament-NF-L(green). The GFAP antibody stains the network of astrocytes in these cultures, while the NF-L antibody stains neurons and their processes. The blue channel shows the localization of DNA. This antibody also works on formalin fixed paraffin embedded brain tissues. Protocol on Datasheet.

Mixed neuron-glial cultures stained with Mouse Monoclonal GFAP, and Chicken Polyclonal Neurofilament-NF-L(green). The GFAP antibody stains the network of astrocytes in these cultures, while the NF-L antibody stains neurons and their processes. The blue channel shows the localization of DNA. This antibody also works on formalin fixed paraffin embedded brain tissues. Protocol on Datasheet.

Strip blot of rat spinal cord protein extract stained with GFAP. A prominent band at about 55 kDa corresponds to the major isoform of GFAP.

Strip blot of rat spinal cord protein extract stained with GFAP. A prominent band at about 55 kDa corresponds to the major isoform of GFAP.

Strip blot of rat spinal cord protein extract stained with GFAP. A prominent band at about 55 kDa corresponds to the major isoform of GFAP.

Strip blot of rat spinal cord protein extract stained with GFAP. A prominent band at about 55 kDa corresponds to the major isoform of GFAP.