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Name Catalog # Size ATPase Na+/K+ transporting alpha 1 MO25023 50 ul Fibrillarin CH22129 100 ul Fibrillarin MO22169 100 µl Fibrillarin RA22147 100 ul Fibrillarin/Nop1p- Nucleolar Marker MO22108 500 ul FluoGreen Tracer SF41000 100 ul GFP GT22101 100 ul Green Fluorescent Protein CH22124 100 ul of 1 mg/ml Green Fluorescent Protein MO22184 100 ul Green Fluorescent Protein (GFP) MO22190 100 ul Green Fluorescent Protein (GFP) RA22134 100 ul of 1 mg/ml High-mobility group protein B1/HMGP1 MO22134 100 ul Lamin A/C CH22136 100 ul Lamin A/C MO22124 100 ul mCherry GT22108 100 ul mCherry MO22192 100 ul mCherry-Chicken CH22115 100 ul mCherry-Rabbit RA22117 100 ul MiTF (Microphthalmia-Associated Transcription Factor) MO47034 0.5 ml Panspecific Nuclear Pore Complex Marker MO22107 500 ul S-Tag/S15 MO22203 100 ul S-Tag/S15 MO22206 100 ul TAF15 MO22114 100 ul Tar DNA Binding Protein 43 (TDP43) MO22117 100 ul
Images
Blot of crude extract of HEK293 cells transfected with pFin-EF1-mCherry vector in lane labelled “+”. The “-” lane is a blot of an equal amount of protein extract from untransfected HEK293 cells. MCA-1C51 binds a major band running at ~28 kDa corresponding to intact full-length mCherry. The two other bands are clearly processed forms of mCherry as they are not present in non-transfected HEK293 cells
Blot of crude extract of HEK293 cells transfected with pFin-EF1-mCherry vector in lane labelled “+”. The “-” lane is a blot of an equal amount of protein extract from untransfected HEK293 cells. MCA-1C51 binds a major band running at ~28 kDa corresponding to intact full-length mCherry. The two other bands are clearly processed forms of mCherry as they are not present in non-transfected HEK293 cells
HEK293 cells transfected with mCherry and visualized in red. The cells were stained with MO22140 in the green channel, and visualized using a confocal microscope. Transfected cells are yellow, showing overlap of the mCherry and the MO22140 antibody. Untransfected HEK293 cells do not express Cherry and do not stain with the antibody, but their nuclei can be visualized using a DNA stain (blue). Blot and transfected cells courtesy of the Semple-Rowland lab at the University of Florida.
HEK293 cells transfected with mCherry and visualized in red. The cells were stained with MO22140 in the green channel, and visualized using a confocal microscope. Transfected cells are yellow, showing overlap of the mCherry and the MO22140 antibody. Untransfected HEK293 cells do not express Cherry and do not stain with the antibody, but their nuclei can be visualized using a DNA stain (blue). Blot and transfected cells courtesy of the Semple-Rowland lab at the University of Florida.