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E18 hippocampal neurons were grown for seven days following the protocol. We fixed and immunostained with MO22145 (green) using our standard immunostaining protocol (on datasheet), chicken antibody to α-internexin (CH22101 red) and for DNA (blue). MO22145 stains the neuronal perikarya and process strongly, and does not stain non neuronal cells in these cultures. The α-internexin antibody stains intermediate or 10nm filament bundles in the cytoplasm of these cells.
E18 hippocampal neurons were grown for seven days following the protocol. We fixed and immunostained with MO22145 (green) using our standard immunostaining protocol (on datasheet), chicken antibody to α-internexin (CH22101 red) and for DNA (blue). MO22145 stains the neuronal perikarya and process strongly, and does not stain non neuronal cells in these cultures. The α-internexin antibody stains intermediate or 10nm filament bundles in the cytoplasm of these cells.