Product Details
Catalog Number: MO22153
Applications: ICC, WB, IHC, IF
Type: Mouse IgG
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Rat
Entrez: 2023
UniProt: P06733
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$325.00Add to Cart

Enolase 1 or α is also known as non-neuronal enolase (NNE) and is expressed in most kinds of tissue, but is absent from neurons. Abnormal expression of NNE is associated with tumor progression in some breast and head and neck cancer. Enolase 2 or γ is also known as neuron specific enolase (NSE), (Check out our Neuron Specific Enolase (NSE)). A switch from NNE to NSE occurs in the development of neurons. Enolase 3 or β is expressed primarily in muscle cells.

Monoclonal antibody MO22153 was raised against the N-terminal 12 amino acids of bovine enolase 1 which was synthesized on a 8-amine lysine core using the multiple antigen presentation method. The antibody was tested for binding to expressed bovine enolase 1, 2 and 3 and shown to be specific for only enolase 1.

Images
NIH/3T3 cells stained with MO22153 (green) and counterstained with chicken polyclonal antibody to vimentin CH22108

NIH/3T3 cells stained with MO22153 (green) and counterstained with chicken polyclonal antibody to vimentin CH22108 (red) and DNA (blue). The MO22153 antibody reveals strong cytoplasmic staining, while the vimentin antibody reveals cytoplasmic intermediate filaments

NIH/3T3 cells stained with MO22153 (green) and counterstained with chicken polyclonal antibody to vimentin CH22108

NIH/3T3 cells stained with MO22153 (green) and counterstained with chicken polyclonal antibody to vimentin CH22108 (red) and DNA (blue). The MO22153 antibody reveals strong cytoplasmic staining, while the vimentin antibody reveals cytoplasmic intermediate filaments