Product Details
Catalog Number: MO22160
Applications: ICC, WB, IHC, IF
Type: Mouse IgG
Immunogen: Full length recombinant human Aurora A protein expressed in and purified from E. coli.
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Mouse, Rat
Entrez: 6790
UniProt: O14965
Downloads: Datasheet (pdf)
Product Sizes
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100 µl$325.00Add to Cart

Aurora proteins are a family of serine/threonine protein kinases that play a key role in the regulation of cell division. Mammalian genomes encode 3 aurora kinases named aurora A, aurora B and aurora C. All 3 contain a regulatory domain at the N terminus which is quite different between the molecules followed by a catalytic serine/threonine kinase domain which is almost identical between them. Aurora A is required for centrosome duplication, entry into mitosis, formation of bipolar spindle and mitotic checkpoint. Aurora B is a chromosomal passenger protein and essential for chromosome condensation, kinetochore functions, spindle checkpoint activation and cytokinesis completion. Aurora C is heavily expressed in testis and is involved in spermatogenesis, but is also expressed in many cell lines and cancer cells and has been less well studied to date.

Images
Aurora A/B Kinase Immunohistochemistry

HeLa cell cultures were stained with MO22160 antibody (green). The antibody stains spindle poles and mitotic spindles at anaphase (top 2 panels) and concentrates on the midbody between the two daughter cells during telophase (bottom 2 panels). It is therefore a useful marker of dividing cells.

Aurora A/B Kinase Immunohistochemistry

HeLa cell cultures were stained with MO22160 antibody (green). The antibody stains spindle poles and mitotic spindles at anaphase (top 2 panels) and concentrates on the midbody between the two daughter cells during telophase (bottom 2 panels). It is therefore a useful marker of dividing cells.

Aurora A/B Kinase Western Blot

Western blot analysis of MO22160 in HeLa cells. Blot of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with MCA-5A12. Nocodazole is a microtubule polymerization inhibitor which induces cells to halt at the G2/M phase and also induces Aurora A expression. The MO22160 monoclonal binds strongly to a band at about 46 kDa, which is Aurora A and also shows binding to a band at 38 kDa.

Aurora A/B Kinase Western Blot

Western blot analysis of MO22160 in HeLa cells. Blot of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with MCA-5A12. Nocodazole is a microtubule polymerization inhibitor which induces cells to halt at the G2/M phase and also induces Aurora A expression. The MO22160 monoclonal binds strongly to a band at about 46 kDa, which is Aurora A and also shows binding to a band at 38 kDa.