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HeLa cell cultures were stained with MO22160 antibody (green). The antibody stains spindle poles and mitotic spindles at anaphase (top 2 panels) and concentrates on the midbody between the two daughter cells during telophase (bottom 2 panels). It is therefore a useful marker of dividing cells.
HeLa cell cultures were stained with MO22160 antibody (green). The antibody stains spindle poles and mitotic spindles at anaphase (top 2 panels) and concentrates on the midbody between the two daughter cells during telophase (bottom 2 panels). It is therefore a useful marker of dividing cells.
Western blot analysis of MO22160 in HeLa cells. Blot of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with MCA-5A12. Nocodazole is a microtubule polymerization inhibitor which induces cells to halt at the G2/M phase and also induces Aurora A expression. The MO22160 monoclonal binds strongly to a band at about 46 kDa, which is Aurora A and also shows binding to a band at 38 kDa.
Western blot analysis of MO22160 in HeLa cells. Blot of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with MCA-5A12. Nocodazole is a microtubule polymerization inhibitor which induces cells to halt at the G2/M phase and also induces Aurora A expression. The MO22160 monoclonal binds strongly to a band at about 46 kDa, which is Aurora A and also shows binding to a band at 38 kDa.