Product Details
Catalog Number: MO22168
Applications: ICC, WB, IHC, IF
Type: Mouse IgG
Dilutions: Immunofluorescent: 1:1,000 Immunocytochemistry: 1:1,000 Immunohistochemistry: 1:1,000 Western Blot: 1:1,000-2,000
Immunogen: Full length human EWS expressed in and purified from E. coli.
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat, Dog, Equine
Entrez: 2130
UniProt: Q01844
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
100 ul of 1 mg/ml$275.00Add to Cart

The Ewing Sarcoma breakpoint region 1 gene EWSR1, was discovered as the name suggests as it is located at the breakpoint on human chromosome 22 which may becomes fused to segments of other chromosomes following chromoplexy, a burst of complex chromosomal rearrangement seen in cancer cells. The genetic rearrangement produces a set of aberrant genes consisting of the 5′ of the EWSR1 gene fused to gene segments of several different transcriptional regulator proteins. The normal EWSR1 gene encodes a protein, EWS RNA binding protein 1, containing an N-terminal transactivation domain followed by a single RRM domain and a single Zinc Finger domain of the ZnF RBZ type. Chromoplexy results in the production of aberrant genes encoding the N-terminal EWS transactivation domain fused to DNA binding segments of various transcription factors, resulting in strong activation of transcription. EWS is an abundant, ubiquitous and multifunctional protein involved in regulating gene expression, cell division, RNA processing and transport. EWS is localized primarily in the nucleus of cells, but has also been found in the cytoplasm, and associated with the plasma membrane in a fashion regulated by the protein kinase PYK2.

Images
Image (No specific type)

An image of a HeLa cell cultures stained with antibody MO22168 (green) to EWS and counterstained with chicken antibody to vimentin (red). Blue is the Hoechst DNA stain. The EWS protein is clearly localized along with the DNA in the nucleus.

Image (No specific type)

An image of a HeLa cell cultures stained with antibody MO22168 (green) to EWS and counterstained with chicken antibody to vimentin (red). Blue is the Hoechst DNA stain. The EWS protein is clearly localized along with the DNA in the nucleus.

Western Blot

Western blot analysis of different cell lines lysates using mouse mAB to EWS, MO22168, dilution 1:1,000 in green: [1] protein standard (red), [2] HeLa, [3] HEK293, [4] mouse NIH-3T3, [5] rat PC12, [6] equine NBL6 and [7] canine A72 cells. The strong band at ~80kDa corresponds to the EWS protein seen in all species tested.

Western Blot

Western blot analysis of different cell lines lysates using mouse mAB to EWS, MO22168, dilution 1:1,000 in green: [1] protein standard (red), [2] HeLa, [3] HEK293, [4] mouse NIH-3T3, [5] rat PC12, [6] equine NBL6 and [7] canine A72 cells. The strong band at ~80kDa corresponds to the EWS protein seen in all species tested.