Product Details
Catalog Number: MO22169
Applications: ICC, WB, IHC, IF
Type: Mouse IgG
Immunogen: Recombinant full length human sequence expressed in and purified from E. coli.
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Mouse, Rat
Entrez: 2091
UniProt: P22087
Downloads: Datasheet (pdf)
Product Sizes
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100 µl$325.00Add to Cart

Nop1p/Fibrillarin was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is 327 amino acids in size (34.5kDa), is essential for yeast viability, and is localized in the nucleoli. Nop1p is the yeast homologue of a protein apparently found in all eukaryotes and archaea generally called fibrillarin. Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6). The HGNC name for this protein is FBL. To raise the fibrillarin antibody, mice were injected with full length recombinant human fibrillarin.

Images
Fibrillarin Immunofluorescence

Immunofluorescent analysis of (A) C6 rat glioma cells and (B) Human embryonic kidney cells stained with mouse mAb to fibrillarin, MO22169, dilution 1:1,000 in red, in both cases costained with chicken pAb to vimentin, CH22108, dilution 1:10,000 in green. The blue is DAPI staining of nuclear DNA.

Fibrillarin Immunofluorescence

Immunofluorescent analysis of (A) C6 rat glioma cells and (B) Human embryonic kidney cells stained with mouse mAb to fibrillarin, MO22169, dilution 1:1,000 in red, in both cases costained with chicken pAb to vimentin, CH22108, dilution 1:10,000 in green. The blue is DAPI staining of nuclear DNA.

Fibrillarin Western Blot

Western blot analysis of different tissue and cell line lysates using mouse mAb to fibrillarin, MO22169. Strong band at ~35kDa corresponds to the fibrillarin protein seen in all cell line lysates. A much weaker band is seen in tissue lysates since fibrillarin is more heavily expressed in rapidly dividing cells.

Fibrillarin Western Blot

Western blot analysis of different tissue and cell line lysates using mouse mAb to fibrillarin, MO22169. Strong band at ~35kDa corresponds to the fibrillarin protein seen in all cell line lysates. A much weaker band is seen in tissue lysates since fibrillarin is more heavily expressed in rapidly dividing cells.