Product Details
Catalog Number: MO22178
Applications: ICC, WB, IF
Type: Mouse IgG
Dilutions: Western Blot: 1:1,000-5,000. Immunocytochemistry/Immunofluorescence: 1:2,000-5,000.
Immunogen: Recombinant construct containing the 2nd, 3rd and 4th Ki67 repeats of the human sequence (amino acids 1,111-1,490) expressed in and purified from E. coli.
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human
Entrez: 4288
UniProt: P46013
Downloads: Datasheet (pdf)
Product Sizes
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100 ug$325.00Add to Cart

The Ki67 proteins were first discovered in an attempt to generate cancer specific monoclonal antibodies. A monoclonal antibody which bound to structures in the nuclei of dividing but not quiescent cells was produced and shown to bind two very large proteins of molecular weight 345kDa and 395kDa. The two proteins were derived from alternate transcripts of a single gene. The presence of Ki67 proteins, detected with an appropriate antibody, is an indicator of cell proliferation and the level of Ki67 expression is one of the most reliable biomarkers of proliferative status of cancer cells.

Images
Ki67 Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with mouse mAb to Ki67, MO22178, dilution 1:2,000 (red). The blue is DAPI staining of nuclear DNA.

Ki67 Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with mouse mAb to Ki67, MO22178, dilution 1:2,000 (red). The blue is DAPI staining of nuclear DNA.

Ki67 Western Blot

Western blot analysis of equal amounts of cell lysates using mouse mAb to Ki67, MO22178, dilution 1:2,000.

Ki67 Western Blot

Western blot analysis of equal amounts of cell lysates using mouse mAb to Ki67, MO22178, dilution 1:2,000.